Anti-Bax antibody [EPR18284] - BSA and Azide free

• WB

Lab

Western blot - Anti-Bax antibody [EPR18284] - BSA and Azide free (AB250635)

This data was developed using the same antibody clone in a different buffer formulation (ab182734).

Lanes 1- 2 : Merged signal (red and green). Green - ab182734 observed at 21 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab182734 was shown to react with Bax in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255363 (knockout cell lysate ab263841) was used. Wild-type HeLa and BAX knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab182734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Bax antibody [EPR18284] (<a href='/en-us/products/primary-antibodies/bax-antibody-epr18284-ab182734'>ab182734</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

BAX knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human BAX knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-bax-knockout-hela-cell-line-ab255363'>ab255363</a>)

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

• WB

Lab

Western blot - Anti-Bax antibody [EPR18284] - BSA and Azide free (AB250635)

This data was developed using the same antibody clone in a different buffer formulation (ab182734).

Lanes 1 - 4 : Merged signal (red and green). Green - ab182734 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab182734 was shown to specifically react with BAX when BAX knockout samples were used. Wild-type and BAX knockout samples were subjected to SDS-PAGE. ab182734 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Bax antibody [EPR18284] - BSA and Azide free (ab250635) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

BAX knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

HT29 whole cell lysate at 20 µg

Predicted band size: 21 kDa

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