AB289364

Anti-Bax antibody [HL236] - BSA and Azide free

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal BAX antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Chinese hamster, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BAX.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue staining for Bax using ab289364 diluted at 1 : 50.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemical analysis of paraffin-embedded rat tissues labelling Bax protein with ab289364 at a 1/100 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa whole cell lysates at 30 µg

Lane 2:

NME4 knockout (KO) HeLa whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

Wild-type (WT) 293T whole cell lysates at 30 µg

Lane 2:

Bax knockout (KO) 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot analysis of various whole cell extracts, separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell extracts at 30 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

All lanes:

Raw264.7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Whole cell extracts separated by 15% SDS-PAGE

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extracts at 30 µg

Lane 2:

Rat2 (Rat fibroblast cell line) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

Mouse spleen tissue extracts at 50 µg

Lane 2:

Rat spleen tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

Wild-type (WT) CHO whole cell lysates at 30 µg

Lane 2:

BAX knockout (KO) CHO whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL236

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BAX. The exact immunogen used to generate this antibody is proprietary information.

Q07812

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.

Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).

See full target information BAX

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:12264-12274 PubMed37934569

2023

Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Tang,Xiuqin Li,Nan Yin,Ming Zhao,Qingchuan Hu,Peiyuan Lv

Bioengineered 13:14426-14437 PubMed36694458

2023

Long non-coding RNA lincRNA-erythroid prosurvival attenuates inflammation by enhancing myosin heavy chain 6 stability through recruitment of heterogeneous nuclear ribonucleoprotein L in myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhen Zhang,Xuejun Kou,Dong Xiao,Zhangjian Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bax antibody [HL236] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise