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AB81083

Anti-Bax antibody [SP47]

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(13 Publications)

Rabbit Recombinant Monoclonal BAX antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 13 publications.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human cervix tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human skin tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human esophagus tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Bax with ab81083 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human skin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling Bax with ab81083 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse skin.

  • Carrier free

    Anti-Bax antibody [SP47] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP47

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Natural products and bioprospecting 15:56 PubMed40839202

2025

Dual inhibition of FAS and HAS2/3 by 4-MU in Realgar-Coptis chinensis unveils a metabolic checkpoint for liver cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Songtao Wu,Yingying Wang,Denghui Deng,Guohua Zheng,Hanxiang Mei,Cong Wang,Xiang Zheng,Chun Gui,Fei Liao,Meixian Xiang

Antioxidants (Basel, Switzerland) 14: PubMed39857399

2025

Chemical Composition of Mexicali Propolis and Its Effect on Gastric Repair in an Indomethacin-Induced Gastric Injury Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Dominguez-Verano,Nadia Jacobo-Herrera,Andrés Castell-Rodríguez,Octavio Canales-Alvarez,Maria Margarita Canales-Martinez,Marco Aurelio Rodriguez-Monroy

Journal of nanobiotechnology 22:251 PubMed38750597

2024

An injectable refrigerated hydrogel for inducing local hypothermia and neuroprotection against traumatic brain injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Han,Zhengzhong Han,Xuyang Huang,Shanshan Li,Guoliang Jin,Junfeng Feng,Decheng Wu,Hongmei Liu

Frontiers in immunology 14:1085456 PubMed37153583

2023

American ginseng with different processing methods ameliorate immunosuppression induced by cyclophosphamide in mice via the MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ting Zhang,Wei Tian,Yu-Shun Lu,Zhi-Man Li,Duo-Duo Ren,Yue Zhang,Ji-Yue Sha,Xiao-Hui Huo,Shan-Shan Li,Yin-Shi Sun

Cells, tissues, organs 212:203-214 PubMed35605590

2022

Developmental Characteristics of the Glomerular and Tubular Portion of the Nephron in the Human Foetal Kidney Cortex: Morphometrical and Immunohistochemical Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Milorad Antic,Marija Dakovic Bjelakovic,Aleksandar Petrovic,Marko Bjelakovic,Goran Radenkovic,Nikola Zivkovic,Miljan Krstic,Vladimir Antic

Aging 13:23603-23619 PubMed34647904

2021

Resveratrol promotes axonal regeneration after spinal cord injury through activating Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zimin Xiang,Shuai Zhang,Xiaodong Yao,Libin Xu,Jianwei Hu,Chenghui Yin,Jianmei Chen,Hao Xu

Journal of ovarian research 14:109 PubMed34454554

2021

LncRNA-MSC-AS1 inhibits the ovarian cancer progression by targeting miR-425-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yinling Zhao,Donglan Yuan,Dandan Zhu,Tianhui Xu,Aihua Huang,Li Jiang,Chiwen Liu,Hua Qian,Xinhua Bu

Molecular and cellular biology 41:e0006521 PubMed34001648

2021

CEBPA-AS1 Knockdown Alleviates Oxygen-Glucose Deprivation/Reperfusion-Induced Neuron Cell Damage by the MicroRNA 24-3p/BOK Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangfu Di,Xinjie Yang,Feng Cheng,Hua Liu,Min Xu

Molecular medicine reports 24: PubMed34036375

2021

lncRNA TUG1 inhibits the cancer stem cell‑like properties of temozolomide‑resistant glioma cells by interacting with EZH2.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiang Cao,Wenying Chai,Yonggang Wang,Dang Tang,Dongchuan Shao,Hai Song,Jiang Long

Cancer biotherapy & radiopharmaceuticals 35:329-337 PubMed32275170

2020

Long Noncoding RNA Activates Wnt/β-Catenin Signaling Pathway in Cervical Cancer Cells by Epigenetically Silencing .

Applications

Unspecified application

Species

Unspecified reactive species

Chi Chi,Min Li,Wenjie Hou,Youguo Chen,Yi Zhang,Jie Chen
View all publications
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