JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB234420

Anti-BAZ2B antibody [EPR20909-88]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal BAZ2B antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse samples.

View Alternative Names

Bromodomain adjacent to zinc finger domain protein 2B, Baz2b

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-BAZ2B antibody [EPR20909-88] (AB234420)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BAZ2B antibody [EPR20909-88] (AB234420)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling BAZ2B with ab234420 at 1/2000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with 3HA-tagged Baz2b expression vector. Anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2μg/ml) dilution.

Flow Cytometry (Intracellular) - Anti-BAZ2B antibody [EPR20909-88] (AB234420)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BAZ2B antibody [EPR20909-88] (AB234420)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with HA-tagged Baz2b expression vector) cells labelling BAZ2B with ab234420 at 1/600 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-BAZ2B antibody [EPR20909-88] (AB234420)
  • WB

Lab

Western blot - Anti-BAZ2B antibody [EPR20909-88] (AB234420)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 62 seconds.

All lanes:

Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420) at 1/500 dilution

All lanes:

Mouse fetal brain at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 240 kDa

Observed band size: 234 kDa

false

Western blot - Anti-BAZ2B antibody [EPR20909-88] (AB234420)
  • WB

Lab

Western blot - Anti-BAZ2B antibody [EPR20909-88] (AB234420)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes.

All lanes:

Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-tag, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with Baz2b expression vector containing a HA-tag, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 240 kDa

Observed band size: 234 kDa

false

  • Carrier free

    Anti-BAZ2B antibody [EPR20909-88] - BSA and Azide free

  • 660 APC

    APC Anti-BAZ2B antibody [EPR20909-88]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-BAZ2B antibody [EPR20909-88]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-BAZ2B antibody [EPR20909-88]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-BAZ2B antibody [EPR20909-88]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-BAZ2B antibody [EPR20909-88]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-BAZ2B antibody [EPR20909-88]

  • 578 PE

    PE Anti-BAZ2B antibody [EPR20909-88]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20909-88

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Lysates need to be freshly made and used for Western blotting immediately to minimize protein degradation.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Regulatory subunit of the ATP-dependent BRF-1 and BRF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (By similarity). Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (By similarity). The BRF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the BRF-5 ISWI chromatin remodeling complex (By similarity). Chromatin reader protein, involved in positively modulating the rate of age-related behavioral deterioration (PubMed : 32103178). Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with histone methyltransferase EHMT1 (PubMed : 32103178).
See full target information Baz2b
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB290639

Anti-BAZ2A/TIP5 antibody [EPR25419-10]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-BAZ2A/TIP5 antibody [EPR25419-10] (AB290639)

  • 0
  • 0
Primary Antibodies

AB238125

Anti-ZNHIT1 antibody [EPR20922-47]

RabMAb|Recombinant|KO Validated|Trial Size|Trial Size

Western blot - Anti-ZNHIT1 antibody [EPR20922-47] (AB238125)

  • 0
  • 0
Primary Antibodies

AB275377

Anti-ALIX antibody [EPR23653-32]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ALIX antibody [EPR23653-32] (AB275377)

  • 5
  • 2
Biochemicals

AB141525

3-Morpholinosydnonimine hydrochloride (SIN-1 hydrochloride), Vasodilator

Chemical Structure - 3-Morpholinosydnonimine hydrochloride (SIN-1 hydrochloride), Vasodilator (AB141525)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com