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AB222890

Anti-BBS1 antibody

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(2 Publications)

Rabbit Polyclonal BBS1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BBS1 aa 200-450.

View Alternative Names

BBS2L2, BBS1, BBSome complex member BBS1, BBS2-like protein 2, Bardet-Biedl syndrome 1 protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS1 antibody (AB222890)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS1 antibody (AB222890)

Paraffin-embedded human kidney tissue stained for BBS1 using ab222890 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-BBS1 antibody (AB222890)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BBS1 antibody (AB222890)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for BBS1 (green) using ab222890 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS1 antibody (AB222890)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS1 antibody (AB222890)

Paraffin-embedded human liver tissue stained for BBS1 using ab222890 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-BBS1 antibody (AB222890)
  • WB

Supplier Data

Western blot - Anti-BBS1 antibody (AB222890)

All lanes:

Western blot - Anti-BBS1 antibody (ab222890) at 1/500 dilution

All lanes:

Mouse lung lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 65 kDa

Observed band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BBS1 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

Q8NFJ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (PubMed : 17574030, PubMed : 22072986). Plays a role in olfactory cilium biogenesis/maintenance and trafficking (By similarity).
See full target information BBS1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental pathology 16:344-351 PubMed38059172

2023

EHD2, a novel HIF target gene, is a promising biomarker in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Chen,Song Xue,Jian Shi,Chunfeng He,Qingchuan Zhang

The Journal of biological chemistry 295:14279-14290 PubMed32759308

2020

The BBSome assembly is spatially controlled by BBS1 and BBS4 in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Avishek Prasai,Marketa Schmidt Cernohorska,Klara Ruppova,Veronika Niederlova,Monika Andelova,Peter Draber,Ondrej Stepanek,Martina Huranova
View all publications
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