Rabbit Recombinant Monoclonal BBS2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--western-blot-img105042.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6178.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128265.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24107.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (AB250371)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization.
Alternative names
BBSome complex member BBS2, Bardet-Biedl syndrome 2 protein, BBS2
Recommended products
Rabbit Recombinant Monoclonal BBS2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13183
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250371 is the carrier-free version of Anti-BBS2 antibody [EPR13183] ab181134.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--western-blot-img105042.jpg "Western blot - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)")
Western blot - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)
This data was developed using Anti-BBS2 antibody [EPR13183] ab181134, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-BBS2 antibody [EPR13183] (Anti-BBS2 antibody [EPR13183] ab181134) at 1/10000 dilution
Lane 1: NTERA2/D1 cell lysate at 20 µg
Lane 2: NCCIT cell lysate at 20 µg
Lane 3: MCF-7 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6178.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)
This data was developed using Anti-BBS2 antibody [EPR13183] ab181134, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling BBS2 with Anti-BBS2 antibody [EPR13183] ab181134 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128265.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)
This data was developed using Anti-BBS2 antibody [EPR13183] ab181134, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling BBS2 with Anti-BBS2 antibody [EPR13183] ab181134 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371), expandable thumbnail](https://content.abcam.com/products/images/bbs2-antibody-epr13183-bsa-and-azide-free-ab250371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24107.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] - BSA and Azide free (ab250371)
This data was developed using Anti-BBS2 antibody [EPR13183] ab181134, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling BBS2 with Anti-BBS2 antibody [EPR13183] ab181134 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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