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AB137507

Anti-BCAR1 antibody

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(1 Publication)

Rabbit Polyclonal BCAR1 antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BCAR1 aa 250-750.

View Alternative Names

CAS, CASS1, CRKAS, BCAR1, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, p130cas

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody (AB137507)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody (AB137507)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for BCAR1 protein at cytoplasm with ab137507 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

Immunohistochemistry (Frozen sections) - Anti-BCAR1 antibody (AB137507)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-BCAR1 antibody (AB137507)

Immunohistochemical analysis of Frozen sectioned adult mouse cerebellum staining p130Cas with ab137507 at a dilution of 1/250 (Green). NF-H, stained by NF-H antibody diluted at 1/500 dilution (Red). The nuclear staining is DAPI (Blue).
Antigen Retrieval : Citrate buffer, pH 6.0, 10 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody (AB137507)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody (AB137507)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for p130Cas protein at cytoplasm with ab137507 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody (AB137507)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAR1 antibody (AB137507)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for p130Cas protein at cytoplasm with ab137507 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-BCAR1 antibody (AB137507)
  • WB

Unknown

Western blot - Anti-BCAR1 antibody (AB137507)

7.5% SDS PAGE

All lanes:

Western blot - Anti-BCAR1 antibody (ab137507) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 93 kDa

false

Western blot - Anti-BCAR1 antibody (AB137507)
  • WB

Unknown

Western blot - Anti-BCAR1 antibody (AB137507)

7.5% SDS PAGE

All lanes:

Western blot - Anti-BCAR1 antibody (ab137507) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human BCAR1 aa 250-750. The exact immunogen used to generate this antibody is proprietary information.

P56945

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed : 12432078, PubMed : 12832404). Implicated in induction of cell migration and cell branching (PubMed : 12432078, PubMed : 12832404, PubMed : 17038317). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).
See full target information BCAR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 76:127-38 PubMed26677978

2015

Identification and Characterization of Tyrosine Kinase Nonreceptor 2 Mutations in Leukemia through Integration of Kinase Inhibitor Screening and Genomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Julia E Maxson,Melissa L Abel,Jinhua Wang,Xianming Deng,Sina Reckel,Samuel B Luty,Huahang Sun,Julie Gorenstein,Seamus B Hughes,Daniel Bottomly,Beth Wilmot,Shannon K McWeeney,Jerald Radich,Oliver Hantschel,Richard E Middleton,Nathanael S Gray,Brian J Druker,Jeffrey W Tyner
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com