AB226914

Anti-BCAR1 (phospho Y165) antibody - N-terminal

Name: Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) | Abcam
Brand: Abcam Limited
SKU: AB226914

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(1 Publication)

Rabbit Polyclonal BCAR1 phospho Y165 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BCAR1 phospho Y165.

View Alternative Names

CAS, CASS1, CRKAS, BCAR1, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, p130cas

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BCAR1 (phospho Y165) (green) using ab226914 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with CIP (calf-intestinal alkaline phosphatase), whole cell extract at 30 µg

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) at 1/10000 dilution

Lane 1:

Untreated (-) HeLa whole cell extract at 30 µg

Lane 2:

HeLa whole cell extract treated with 100µM Pervanadate, 1hr at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) at 1/500 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

C8D30 whole cell lysates at 30 µg

Lane 3:

NIH 3T3 whole cell lysates at 30 µg

Lane 4:

Raw264.7 whole cell lysates at 30 µg

Lane 5:

C2C12 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) at 1/500 dilution

Lane 1:

PC-12 cell lysates at 30 µg

Lane 2:

Rat2 cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (AB226914)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) at 1/20000 dilution

Lane 1:

Untreated (-) NIH 3T3 whole cell extract at 30 µg

Lane 2:

NIH 3T3 whole cell extract treated with 1mM pervanadate, 30mins at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human BCAR1 phospho Y165. The exact immunogen used to generate this antibody is proprietary information.

P56945

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed : 12432078, PubMed : 12832404). Implicated in induction of cell migration and cell branching (PubMed : 12432078, PubMed : 12832404, PubMed : 17038317). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).

See full target information BCAR1 phospho Y165

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39272974

2024

The Expression of Toll-like Receptors in Cartilage Endplate Cells: A Role of Toll-like Receptor 2 in Pro-Inflammatory and Pro-Catabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Mengis,Laura Bernhard,Andrea Nüesch,Irina Heggli,Nick Herger,Jan Devan,Roy Marcus,Christoph J Laux,Florian Brunner,Mazda Farshad,Oliver Distler,Christine L Le Maitre,Stefan Dudli

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