AB41827

Anti-BCAR1 (phospho Y762) antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal BCAR1 phospho Y762 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Bcar1 pY762.

View Alternative Names

Cas, Crkas, Bcar1, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, p130cas

1 Images

Western blot - Anti-BCAR1 (phospho Y762) antibody (AB41827)

WB

Unknown

Western blot - Anti-BCAR1 (phospho Y762) antibody (AB41827)

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-BCAR1 (lanes 1 & 2) or anti-BCAR1 (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-BCAR1 (Tyr-751) peptide (lane 6).

All lanes:

Western blot - Anti-BCAR1 (phospho Y762) antibody (ab41827)

Predicted band size: 93 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Synthetic Peptide within Rat Bcar1 pY762. The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated BCAR1 on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-BCAR1(Tyr-751) peptide before affinity purification using phospho-BCAR1(Tyr-762) peptide (without carrier).

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (By similarity). Implicated in induction of cell migration and cell branching (By similarity). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).

See full target information Bcar1 pY762

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 287:148-158 PubMed22084245

2011

Protein-tyrosine kinase 6 promotes peripheral adhesion complex formation and cell migration by phosphorylating p130 CRK-associated substrate.

Applications

ICC/IF

Species

Human

Yu Zheng,John M Asara,Angela L Tyner

View all publications

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Product promise

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