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AB71162

Anti-BCAS3 antibody

0

(1 Review)

|

(2 Publications)

Rabbit Polyclonal BCAS3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BCAS3.

View Alternative Names

BCAS3 microtubule associated cell migration factor, Breast carcinoma-amplified sequence 3, GAOB1, BCAS3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (AB71162)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (AB71162)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-BCAS3 antibody (AB71162)
  • IP

Unknown

Immunoprecipitation - Anti-BCAS3 antibody (AB71162)

Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
Lane 1 : ab71162 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

All lanes : Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded.
Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-BCAS3 antibody (ab71162)

Predicted band size: 101 kDa

false

Western blot - Anti-BCAS3 antibody (AB71162)
  • WB

Unknown

Western blot - Anti-BCAS3 antibody (AB71162)

All lanes:

Western blot - Anti-BCAS3 antibody (ab71162) at 0.04 µg/mL

Lane 1:

Whole cell lysate from MCF7 cells at 50 µg

Lane 2:

Whole cell lysate from MCF7 cells at 15 µg

Lane 3:

Whole cell lysate from MCF7 cells at 5 µg

Predicted band size: 101 kDa

Observed band size: 117 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human BCAS3. The exact immunogen used to generate this antibody is proprietary information.

Q9H6U6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation (By similarity). Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin. Plays a regulatory role in autophagic activity. In complex with PHAF1, associates with the preautophagosomal structure during both non-selective and selective autophagy (PubMed : 33499712). Probably binds phosphatidylinositol 3-phosphate (PtdIns3P) which would mediate the recruitment preautophagosomal structures (PubMed : 33499712).
See full target information BCAS3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38869949

2024

C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Anais Franco-Romero,Valeria Morbidoni,Giulia Milan,Roberta Sartori,Jesper Wulff,Vanina Romanello,Andrea Armani,Leonardo Salviati,Maria Conte,Stefano Salvioli,Claudio Franceschi,Viviana Buonomo,Casey O Swoboda,Paolo Grumati,Luca Pannone,Simone Martinelli,Harold Bj Jefferies,Ivan Dikic,Jennifer van der Laan,Filipe Cabreiro,Douglas P Millay,Sharon A Tooze,Eva Trevisson,Marco Sandri

Frontiers in molecular neuroscience 15:1110986 PubMed36733270

2023

Inhibitory synapse dysfunction and epileptic susceptibility associated with KIF2A deletion in cortical interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Nuria Ruiz-Reig,Dario García-Sánchez,Olivier Schakman,Philippe Gailly,Fadel Tissir
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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