Anti-BCAT1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB125209)

Immunohistochemical analysis of paraffin-embedded Rat brain tissue tissue labeling BCAT1 with ab125209 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody, ab125209, overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB125209)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue tissue labeling BCAT1 with ab125209 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody, ab125209, overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• WB

Supplier Data

Western blot - Anti-BCAT1 antibody (AB125209)

Blocking and diluting buffer and concentration : 5% non-fat milk/TBS

All lanes:

Western blot - Anti-BCAT1 antibody (ab125209) at 0.5 µg/mL

Lane 1:

Human Raji whole cell lysates at 30 µg

Lane 2:

Human Jurkat whole cell lysates at 30 µg

Lane 3:

Human MOLT-4 whole cell lysates at 30 µg

Lane 4:

Human Hela whole cell lysates at 30 µg

Lane 5:

Rat brain tissue lysates at 30 µg

Lane 6:

Rat kidney tissue lysates at 30 µg

Lane 7:

mouse brain tissue lysates at 30 µg

Lane 8:

Mouse kidney tissue lysates at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Exposure time: 2hr