AB232706

Anti-BCAT1 antibody

Name: Anti-BCAT1 antibody (ab232706) | Abcam
Brand: Abcam Limited
SKU: AB232706
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal BCAT1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Bcat1 aa 150 to C-terminus.

View Alternative Names

Eca39, Bcat1, BCAT(c)

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

Formalin-fixed, paraffin-embedded rat heart tissue stained for BCAT1 using ab232706 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

Formalin-fixed, paraffin-embedded rat testis tissue stained for BCAT1 using ab232706 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

Formalin-fixed, paraffin-embedded rat brain tissue stained for BCAT1 using ab232706 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-BCAT1 antibody (AB232706)

All lanes:

Western blot - Anti-BCAT1 antibody (ab232706) at 1 µg/mL

All lanes:

Mouse heart lysate

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-BCAT1 antibody (AB232706)

All lanes:

Western blot - Anti-BCAT1 antibody (ab232706) at 1 µg/mL

All lanes:

Mouse cerebrum lysate

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-BCAT1 antibody (AB232706)

All lanes:

Western blot - Anti-BCAT1 antibody (ab232706) at 1 µg/mL

All lanes:

Rat cerebrum lysate

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-BCAT1 antibody (AB232706)

All lanes:

Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (<a href='/en-us/products/unavailable/calcium-independent-phospholipase-a2pla2g6-antibody-ab23706'>ab23706</a>) at 1 µg/mL

All lanes:

Recombinant rat BCAT1 protein

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Bcat1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54690

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

See full target information Bcat1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:327 PubMed39716292

2024

Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Guanjin Shi,Yanyan Ge,Shanshan Huang,Ningning Cui,Le Tan,Rui Liu,Xuefeng Yang

Acta diabetologica 62:227-240 PubMed39150511

2024

Accumulation of branched-chain amino acids deteriorates the neuroinflammatory response of Müller cells in diabetic retinopathy via leucine/Sestrin2-mediated sensing of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Gong,Jingyi Wang,Dawei Luo,Yupeng Xu,Rulin Zhang,Xin Li,Zihan Yin,Junwei Fang,Haiyan Wang

Nature communications 13:7578 PubMed36481721

2022

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Chen,Chunyu Yu,Xinhua Liu,Beibei Liu,Xiaodi Wu,Jiajing Wu,Dong Yan,Lulu Han,Zifan Tang,Xinyi Yuan,Jianqiu Wang,Yue Wang,Shumeng Liu,Lin Shan,Yongfeng Shang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCAT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (AB232706)

Western blot - Anti-BCAT1 antibody (AB232706)

Western blot - Anti-BCAT1 antibody (AB232706)

Western blot - Anti-BCAT1 antibody (AB232706)

Western blot - Anti-BCAT1 antibody (AB232706)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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