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Rabbit Recombinant Monoclonal BCAT1 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.

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Images

Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (AB320651), expandable thumbnail
  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (AB320651), expandable thumbnail
  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (AB320651), expandable thumbnail
  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (AB320651), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human, Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Alternative names

BCT1, ECA39, BCAT1, BCAT(c)

Recommended products

Rabbit Recombinant Monoclonal BCAT1 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR29208-26
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab320651 is the carrier-free version of Anti-BCAT1 antibody [EPR29208-26] ab320650.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651), expandable thumbnail

    Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651)

    This data was developed using Anti-BCAT1 antibody [EPR29208-26] ab320650, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands higher than 50 kDa and lower than 37 kDa are unknown.

    All lanes: Western blot - Anti-BCAT1 antibody [EPR29208-26] (Anti-BCAT1 antibody [EPR29208-26] ab320650) at 1/1000 dilution

    Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 2: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa

    Exposure time: 15s

  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651), expandable thumbnail

    Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651)

    This data was developed using Anti-BCAT1 antibody [EPR29208-26] ab320650, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human BCAT2.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-BCAT1 antibody [EPR29208-26] (Anti-BCAT1 antibody [EPR29208-26] ab320650) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

    Lane 2: 293T cells transfected with a human BCAT2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

    Lane 3: 293T cells transfected with a human BCAT1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651), expandable thumbnail

    Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651)

    This data was developed using Anti-BCAT1 antibody [EPR29208-26] ab320650, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands higher than 50 kDa and lower than 37 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 103 seconds; Lane 3: 15 seconds.

    All lanes: Western blot - Anti-BCAT1 antibody [EPR29208-26] (Anti-BCAT1 antibody [EPR29208-26] ab320650) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting BCAT1 whole cell lysate at 20 µg

    Lane 3: MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 36 kDa

  • Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651), expandable thumbnail

    Western blot - Anti-BCAT1 antibody [EPR29208-26] - BSA and Azide free (ab320651)

    This data was developed using Anti-BCAT1 antibody [EPR29208-26] ab320650, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: heart, skeletal muscle, liver (PMID:31435015).

    The identity of the bands higher than 50 kDa and lower than 37 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-BCAT1 antibody [EPR29208-26] (Anti-BCAT1 antibody [EPR29208-26] ab320650) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 6: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 36 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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