AB233650

Anti-BCAT2 antibody [7G3A11]

Name: Anti-BCAT2 antibody [7G3A11] (ab233650) | Abcam
Brand: Abcam Limited
SKU: AB233650

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(1 Publication)

Mouse Monoclonal BCAT2 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BCAT2 aa 250 to C-terminus.

View Alternative Names

BCATM, BCT2, ECA40, BCAT2, BCAT(m), Placental protein 18, PP18

3 Images

Flow Cytometry - Anti-BCAT2 antibody [7G3A11] (AB233650)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-BCAT2 antibody [7G3A11] (AB233650)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BCAT2 using ab233650 at 1/200 dilution (green) compared to the negative control (red).

Supplier Data

Western blot - Anti-BCAT2 antibody [7G3A11] (AB233650)

All lanes:

Western blot - Anti-BCAT2 antibody [7G3A11] (ab233650) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

BCAT2 (amino acids 259-393)-hIgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-BCAT2 antibody [7G3A11] (AB233650)

(Expected MW is 41.5 kDa)

All lanes:

Western blot - Anti-BCAT2 antibody [7G3A11] (ab233650) at 1/500 dilution

All lanes:

Human BCAT2 recombinant protein (amino acids 259-393)

Predicted band size: 44 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G3A11

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human BCAT2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15382

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed : 17050531, PubMed : 25653144, PubMed : 8702755). Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes (By similarity). Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning (By similarity). May also function as a transporter of branched chain alpha-keto acids (By similarity).

See full target information BCAT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23237 PubMed36193555

2022

ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guanli Zhang,Yan Feng,Min Wang,Xin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCAT2 antibody [7G3A11]

Flow Cytometry - Anti-BCAT2 antibody [7G3A11] (AB233650)

Western blot - Anti-BCAT2 antibody [7G3A11] (AB233650)

Western blot - Anti-BCAT2 antibody [7G3A11] (AB233650)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise