AB95976

Anti-BCAT2 antibody

Name: Anti-BCAT2 antibody (ab95976) | Abcam
Brand: Abcam Limited
SKU: AB95976
Price: 230 USD
Availability: InStock

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(12 Publications)

Rabbit Polyclonal BCAT2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BCAT2 aa 1-350.

View Alternative Names

BCATM, BCT2, ECA40, BCAT2, BCAT(m), Placental protein 18, PP18

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (AB95976)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (AB95976)

Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/500 dilution in immunohistochemical analysis. Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supplier Data

Immunoprecipitation - Anti-BCAT2 antibody (AB95976)

BCAT2 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab307833 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307833 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : MCF7 whole cell lysate 10 ug Lane 2 : ab307833 IP in MCF7 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307833 in MCF7 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : Lanes 1-3 : 6 seconds (left), Lanes 1-3 : 180 seconds (right). The IP experiment was performed by ab307833 using MCF cells. On the left the IP blot was probed with ab307833 and on the right the blot was probed by another anti-BCAT2 antibody (ab95976)(1 : 1000 dilution).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (AB95976)

Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-BCAT2 antibody (AB95976)

All lanes:

Western blot - Anti-BCAT2 antibody (ab95976) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human BCAT2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

O15382

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed : 17050531, PubMed : 25653144, PubMed : 8702755). Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes (By similarity). Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning (By similarity). May also function as a transporter of branched chain alpha-keto acids (By similarity).

See full target information BCAT2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:327 PubMed39716292

2024

Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Guanjin Shi,Yanyan Ge,Shanshan Huang,Ningning Cui,Le Tan,Rui Liu,Xuefeng Yang

eLife 12: PubMed39226092

2024

A 2-hydroxybutyrate-mediated feedback loop regulates muscular fatigue.

Applications

Unspecified application

Species

Unspecified reactive species

Brennan J Wadsworth,Marina Leiwe,Eleanor A Minogue,Pedro P Cunha,Viktor Engman,Carolin Brombach,Christos Asvestis,Shiv K Sah-Teli,Emilia Marklund,Peppi Koivunen,Jorge L Ruas,Helene Rundqvist,Johanna T Lanner,Randall S Johnson

Acta diabetologica 62:227-240 PubMed39150511

2024

Accumulation of branched-chain amino acids deteriorates the neuroinflammatory response of Müller cells in diabetic retinopathy via leucine/Sestrin2-mediated sensing of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Gong,Jingyi Wang,Dawei Luo,Yupeng Xu,Rulin Zhang,Xin Li,Zihan Yin,Junwei Fang,Haiyan Wang

Nature communications 14:1595 PubMed36949106

2023

A defect in mitochondrial protein translation influences mitonuclear communication in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Tian Liang,Yao Wei Lu,Xuyang Fu,Xiaoxuan Dong,Linbin Pu,Tingting Hong,Yuxia Zhou,Yu Zhang,Ning Liu,Feng Zhang,Jianming Liu,Andrea P Malizia,Hong Yu,Wei Zhu,Douglas B Cowan,Hong Chen,Xinyang Hu,John D Mably,Jian'an Wang,Da-Zhi Wang,Jinghai Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2207155 PubMed36642843

2023

BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Cai,Jinbo Chen,Zhengzheng Yu,Huihuang Li,Zhi Liu,Dingshan Deng,Jinhui Liu,Chunliang Chen,Chunyu Zhang,Zhenyu Ou,Minfeng Chen,Jiao Hu,Xiongbing Zu

Cells 11: PubMed36359919

2022

Branched-Chain Amino Acids Are Linked with Alzheimer's Disease-Related Pathology and Cognitive Deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Md Abu Bakkar Siddik,Caitlyn A Mullins,Alyssa Kramer,Harsh Shah,Ritchel B Gannaban,Masoud Zabet-Moghaddam,Ryan M Huebinger,Vijay K Hegde,Sheba M J MohanKumar,Puliyur S MohanKumar,Andrew C Shin

Frontiers in cell and developmental biology 10:816249 PubMed35265615

2022

Identification of 14 Differentially-Expressed Metabolism-Related Genes as Potential Targets of Gastric Cancer by Integrated Proteomics and Transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxin Zhang,Wenwei Liu,Wei Feng,Xiaofeng Wang,Tianxiang Lei,Zehong Chen,Wu Song

Cell death and differentiation 28:1222-1236 PubMed33097833

2020

Branched-chain amino acid aminotransferase 2 regulates ferroptotic cell death in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Wang,Zhengyang Zhang,Hsiang-I Tsai,Yanfang Liu,Jie Gao,Ming Wang,Lian Song,Xiongfeng Cao,Zhanxue Xu,Hongbo Chen,Aihua Gong,Dongqing Wang,Fang Cheng,Haitao Zhu

Diabetes, metabolic syndrome and obesity : targets 11:65-84 PubMed29618935

2018

Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Rajan Singh,Shehla Pervin,Se-Jin Lee,Alan Kuo,Victor Grijalva,John David,Laurent Vergnes,Srinivasa T Reddy

Scientific reports 7:7820 PubMed28798381

2017

Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism.

Applications

Species

Human

Ieva Antanavičiūtė,Valeryia Mikalayeva,Ieva Ceslevičienė,Gintarė Milašiūtė,Vytenis Arvydas Skeberdis,Sergio Bordel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCAT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (AB95976)

Immunoprecipitation - Anti-BCAT2 antibody (AB95976)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (AB95976)

Western blot - Anti-BCAT2 antibody (AB95976)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

Product promise