Rabbit Polyclonal BCAT2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BCAT2 aa 1-350.
Images
Publications
Filter by
- Nature communications 14:15952023A defect in mitochondrial protein translation influences mitonuclear communication in the heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Gao,Tian Liang,Yao Wei Lu,Xuyang Fu,Xiaoxuan Dong,Linbin Pu,Tingting Hong,Yuxia Zhou,Yu Zhang,Ning Liu,Feng Zhang,Jianming Liu,Andrea P Malizia,Hong Yu,Wei Zhu,Douglas B Cowan,Hong Chen,Xinyang Hu,John D Mably,Jian'an Wang,Da-Zhi Wang,Jinghai ChenPubMed 36949106
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22071552023BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyong Cai,Jinbo Chen,Zhengzheng Yu,Huihuang Li,Zhi Liu,Dingshan Deng,Jinhui Liu,Chunliang Chen,Chunyu Zhang,Zhenyu Ou,Minfeng Chen,Jiao Hu,Xiongbing ZuPubMed 36642843
- Cells 11:2022Branched-Chain Amino Acids Are Linked with Alzheimer's Disease-Related Pathology and Cognitive Deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Abu Bakkar Siddik,Caitlyn A Mullins,Alyssa Kramer,Harsh Shah,Ritchel B Gannaban,Masoud Zabet-Moghaddam,Ryan M Huebinger,Vijay K Hegde,Sheba M J MohanKumar,Puliyur S MohanKumar,Andrew C ShinPubMed 36359919
- Frontiers in cell and developmental biology 10:8162492022Identification of 14 Differentially-Expressed Metabolism-Related Genes as Potential Targets of Gastric Cancer by Integrated Proteomics and Transcriptomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongxin Zhang,Wenwei Liu,Wei Feng,Xiaofeng Wang,Tianxiang Lei,Zehong Chen,Wu SongPubMed 35265615
- Cell death and differentiation 28:1222-12362020Branched-chain amino acid aminotransferase 2 regulates ferroptotic cell death in cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKang Wang,Zhengyang Zhang,Hsiang-I Tsai,Yanfang Liu,Jie Gao,Ming Wang,Lian Song,Xiongfeng Cao,Zhanxue Xu,Hongbo Chen,Aihua Gong,Dongqing Wang,Fang Cheng,Haitao ZhuPubMed 33097833
- Diabetes, metabolic syndrome and obesity : targets 11:65-842018Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajan Singh et. al.PubMed 29618935
- Scientific reports 7:78202017Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism.
- Frontiers in cellular neuroscience 11:962017MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway.
- PloS one 9:e1026152014Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes.Applications:WBReactive species:MouseAbderrazak Kitsy,Skyla Carney,Juan C Vivar,Megan S Knight,Mildred A Pointer,Judith K Gwathmey,Sujoy GhoshPubMed 25050624
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BCAT2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link O15382
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:17050531, PubMed:25653144, PubMed:8702755). May also function as a transporter of branched chain alpha-keto acids (By similarity).
Alternative names
BCATM, BCT2, ECA40, BCAT2, BCAT(m), Placental protein 18, PP18
Recommended products
Rabbit Polyclonal BCAT2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BCAT2 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BCAT2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link O15382
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (ab95976)
Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (ab95976)
Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western blot - Anti-BCAT2 antibody (ab95976)
All lanes: Western blot - Anti-BCAT2 antibody (ab95976) at 1/1000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Observed band size: 44 kDa
Immunoprecipitation - Anti-BCAT2 antibody (ab95976)
BCAT2 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with Anti-BCAT2 antibody [EPR27488-72] ab307833 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-BCAT2 antibody [EPR27488-72] ab307833 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 whole cell lysate 10 ug
Lane 2: Anti-BCAT2 antibody [EPR27488-72] ab307833 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BCAT2 antibody [EPR27488-72] ab307833 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-3: 6 seconds (left), Lanes 1-3: 180 seconds (right).
The IP experiment was performed by Anti-BCAT2 antibody [EPR27488-72] ab307833 using MCF cells. On the left the IP blot was probed with Anti-BCAT2 antibody [EPR27488-72] ab307833 and on the right the blot was probed by another anti-BCAT2 antibody (ab95976)(1:1000 dilution).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com