JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122599

Anti-BCDIN3D antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal BCDIN3D antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BCDIN3D.

View Alternative Names

RNA 5'-monophosphate methyltransferase, BCDIN3 domain-containing protein, BCDIN3D

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-BCDIN3D antibody (AB122599)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BCDIN3D antibody (AB122599)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122599 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCDIN3D antibody (AB122599)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCDIN3D antibody (AB122599)

ab122599, at 1/50 dilution, staining BCDIN3D in paraffin embedded Human cerebellum tissue by Immunohistochemistry.

Western blot - Anti-BCDIN3D antibody (AB122599)
  • WB

Unknown

Western blot - Anti-BCDIN3D antibody (AB122599)

All lanes:

Western blot - Anti-BCDIN3D antibody (ab122599) at 1/250 dilution

Lane 1:

RT 4 lysate

Lane 2:

U 251 MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 33 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BCDIN3D. The exact immunogen used to generate this antibody is proprietary information.

Q7Z5W3

style
left-column

Complementary Products

Proteins & Peptides

AB134588

Recombinant Human BCDIN3D protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human BCDIN3D protein (His tag N-Terminus) (AB134588)

  • 0
  • 0
Primary Antibodies

AB317508

Anti-CXCL13 antibody [RM1170]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [RM1170] (AB317508)

  • 0
  • 0
Primary Antibodies

AB254324

Anti-IGFBP5 antibody [EPR18013-137]

RabMAb|Recombinant

Immunoprecipitation - Anti-IGFBP5 antibody [EPR18013-137] (AB254324)

  • 0
  • 0
Primary Antibodies

AB134968

Anti-BRMS1 antibody [EPR7202]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody [EPR7202] (AB134968)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store undiluted
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1 (PubMed : 28119416, PubMed : 31329584, PubMed : 31919512). Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing (PubMed : 23063121, PubMed : 28119416). The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing : methylation at this position reduces the processing of pre-miRNAs by DICER1 (PubMed : 23063121). Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145 (PubMed : 23063121, PubMed : 28119416).
See full target information BCDIN3D
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3793 PubMed38714822

2024

An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen M Coscia,Andrew S Moore,Cameron P Thompson,Christian F Tirrito,E Michael Ostap,Erika L F Holzbaur
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com