Anti-BCDIN3D antibody [EPR28761-82] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BCDIN3D antibody [EPR28761-82] - BSA and Azide free (AB317623)

This data was developed using ab317622, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) cells labelling BCDIN3D with ab317622 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-BCDIN3D antibody [EPR28761-82] - BSA and Azide free (AB317623)

This data was developed using ab317622, the same antibody clone in a different buffer formulation.

The identity of the higher MW band at approximately 50 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-BCDIN3D antibody [EPR28761-82] (<a href='/en-us/products/primary-antibodies/bcdin3d-antibody-epr28761-82-ab317622'>ab317622</a>) at 1/1000 dilution

All lanes:

Human lung cancer tissue at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-BCDIN3D antibody [EPR28761-82] - BSA and Azide free (AB317623)

This data was developed using ab317622, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-BCDIN3D antibody [EPR28761-82] (<a href='/en-us/products/primary-antibodies/bcdin3d-antibody-epr28761-82-ab317622'>ab317622</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 50 µg

Lane 5:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-BCDIN3D antibody [EPR28761-82] - BSA and Azide free (AB317623)

This data was developed using ab317622, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-BCDIN3D antibody [EPR28761-82] (<a href='/en-us/products/primary-antibodies/bcdin3d-antibody-epr28761-82-ab317622'>ab317622</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

293T transfected with siRNA specifically targeting BCDIN3D whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa,36 kDa

true

Exposure time: 180s