Anti-BCG1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCG1 antibody (AB236592)

Paraffin-embedded human testis tissue stained for BCG1 using ab236592 at 1/320 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCG1 antibody (AB236592)

Paraffin-embedded human adrenal gland tissue stained for BCG1 using ab236592 at 1/320 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BCG1 antibody (AB236592)

4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BCG1 (green) using ab236592 at 1/145 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

• IP

Supplier Data

Immunoprecipitation - Anti-BCG1 antibody (AB236592)

BCG1 was immunoprecipitated from 500 μg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236592 at 1/200 dilution.

Lane 1 : Rabbit control IgG IP in HepG2 whole cell lysate.

Lane 2 : ab236592 IP in HepG2 whole cell lysate.

Lane 3 : HepG2 whole cell lysate 20 μg (Input).

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-BCG1 antibody (ab236592)

Predicted band size: 65 kDa

false

• WB

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Western blot - Anti-BCG1 antibody (AB236592)

All lanes:

Western blot - Anti-BCG1 antibody (ab236592) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 4:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 5:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 65 kDa

false