Rabbit Recombinant Monoclonal BCKDHA antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunoprecipitation-img406391.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169)")
![Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--western-blot-img405427.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169)")
![Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--western-blot-img405426.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405424.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (AB305169)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.
Alternative names
Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA
Recommended products
Rabbit Recombinant Monoclonal BCKDHA antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27003-11
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunoprecipitation-img406391.jpg "Immunoprecipitation - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Immunoprecipitation - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using Anti-BCKDHA antibody [EPR27003-11] ab305168, the same antibody clone in a different buffer formulation.
BCKDHA was immunoprecipitated from 0.35 mg HEK293T (human embryonic kidney epithelial cell), whole cell lysate 10 ug with Anti-BCKDHA antibody [EPR27003-11] ab305168 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-BCKDHA antibody [EPR27003-11] ab305168 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK293T (human embryonic kidney epithelial cell), whole cell lysate 10 ug
Lane 2: ABAB305168 IP in HEK293T whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BCKDHA antibody [EPR27003-11] ab305168 in HEK293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 secondsAll lanes: Immunoprecipitation - Anti-BCKDHA antibody [EPR27003-11] (Anti-BCKDHA antibody [EPR27003-11] ab305168) at 1/1000 dilution
Lane 1: HEK293T (human embryonic kidney epithelial cell), whole cell lysate 10 μg
Lane 2: HEK293T whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 49 kDa
Exposure time: 15s
![Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--western-blot-img405427.jpg "Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using 305168, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: In Western blot, Anti-BCKDHA antibody [EPR27003-11] ab305168 was shown to bind specifically to BCKDHA. A band was observed at 49 kDa in wild-type HEK293T cell lysates with no signal observed at this size in BCKDHA knockout cell line Human BCKDHA knockout HEK-293T cell line ab266439 (knockout cell lysate Human BCKDHA knockout HEK-293T cell lysate ab258324).
15 seconds
Exposure time:All lanes: Western blot - Anti-BCKDHA antibody [EPR27003-11] (Anti-BCKDHA antibody [EPR27003-11] ab305168) at 1/1000 dilution
Lane 1: Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate 20 μg
Lane 2: BCKDHA knockout HEK293T whole cell lysate 20 μg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 49 kDa
Exposure time: 15s
![Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--western-blot-img405426.jpg "Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Western blot - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using 305168, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
26 seconds
Exposure time:All lanes: Western blot - Anti-BCKDHA antibody [EPR27003-11] (Anti-BCKDHA antibody [EPR27003-11] ab305168) at 1/1000 dilution
All lanes: Human liver tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 49 kDa
Exposure time: 26s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405424.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using Anti-BCKDHA antibody [EPR27003-11] ab305168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling BCKDHA with Anti-BCKDHA antibody [EPR27003-11] ab305168 at 1/2000 (0.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human liver (PMID: 3224821). The section was incubated with Anti-BCKDHA antibody [EPR27003-11] ab305168 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using Anti-BCKDHA antibody [EPR27003-11] ab305168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colorectal can tissue labeling BCKDHA with Anti-BCKDHA antibody [EPR27003-11] ab305168 at 1/2000 (0.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human colorectal cancer (PMID: 28501528). The section was incubated with Anti-BCKDHA antibody [EPR27003-11] ab305168 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405422.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using Anti-BCKDHA antibody [EPR27003-11] ab305168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BCKDHA with Anti-BCKDHA antibody [EPR27003-11] ab305168 at 1/2000 (0.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human colon (PMID: 28501528). The section was incubated with Anti-BCKDHA antibody [EPR27003-11] ab305168 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405421.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)
This data was developed using Anti-BCKDHA antibody [EPR27003-11] ab305168, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 tissue labeling BCKDHA with Anti-BCKDHA antibody [EPR27003-11] ab305168 at 1/5000 (0.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on (A) Wild-type HEK293T cell pellet; no staining on (B) BCKDHA knockout HEK293T (Human BCKDHA knockout HEK-293T cell line ab266439) cell pellet.The section was incubated with Anti-BCKDHA antibody [EPR27003-11] ab305168 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405422.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169), expandable thumbnail](https://content.abcam.com/products/images/bckdha-antibody-epr27003-11-bsa-and-azide-free-ab305169--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405421.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody [EPR27003-11] - BSA and Azide free (ab305169)")
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