Rabbit Recombinant Monoclonal BCKDHA phospho S292 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human BCKDHA phospho S292.
Images
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img105541.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)")
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437614.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)")
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437615.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)")
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437617.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)")
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437616.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99.81% Borate buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human BCKDHA phospho S292. The exact immunogen used to generate this antibody is proprietary information. Database link P12694
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.
Alternative names
Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA
Recommended products
Rabbit Recombinant Monoclonal BCKDHA phospho S292 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human BCKDHA phospho S292.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99.81% Borate buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human BCKDHA phospho S292. The exact immunogen used to generate this antibody is proprietary information. Database link P12694
- Clone number
- BLR060F
- Concentration
- Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img105541.jpg "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)")
Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
Chemiluminescence with an exposure time of 30 seconds.
Lower Panel: Rabbit anti-RPL10
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5: LNCaP (human prostate cancer cell line) whole cell lysate
Lane 6: GaMG whole cell lysate
Lane 7: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 8: MCF-7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 9: Differentiated 3T3-L1 whole cell lysate
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 50 kDa
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437614.jpg "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)")
Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
BCKDHA (phospho S292) Western blot staining using rabbit Anti-BCKDHA (phospho S292) antibody
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution
Lane 1: HEK293T cells mock treated (-) whole cell lysate at 50 µg
Lane 2: HEK293T cells treated with phosphatases (+) whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 49 kDa
Exposure time: 3min
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437615.jpg "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)")
Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
BCKDHA (phospho S292) Western blot staining using rabbit Anti-BCKDHA (phospho S292) antibody
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 50 µg
Lane 2: CT26 whole cell lysate at 50 µg
Lane 3: TCMK-1 whole cell lysate at 50 µg
Lane 4: BW5147.3 whole cell lysate at 50 µg
Lane 5: CH27 whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 49 kDa
Exposure time: 75s
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437617.jpg "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)")
Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/10 dilution
All lanes: NIH 3T3 whole cell lysate at 0.4 mg/mL
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 49 kDa
![Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961), expandable thumbnail](https://content.abcam.com/products/images/bckdha-phospho-s292-antibody-blr060f-ab275961--western-blot-img437616.jpg "Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)")
Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/10 dilution
All lanes: HEK293T whole cell lysate at 0.08 mg/mL
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 49 kDa
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