AB275961

Anti-BCKDHA (phospho S292) antibody [BLR060F]

20ul selling size
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal BCKDHA phospho S292 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BCKDHA phospho S292.

View Alternative Names

Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA

5 Images

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel : Rabbit anti-RPL10

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 5:

LNCaP (human prostate cancer cell line) whole cell lysate

Lane 6:

GaMG whole cell lysate

Lane 7:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 8:

MCF-7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 9:

Differentiated 3T3-L1 whole cell lysate

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

HEK293T cells mock treated (-) whole cell lysate at 50 µg

Lane 2:

HEK293T cells treated with phosphatases (+) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/10 dilution

All lanes:

HEK293T whole cell lysate at 0.08 mg/mL

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/10 dilution

All lanes:

NIH 3T3 whole cell lysate at 0.4 mg/mL

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

CT26 whole cell lysate at 50 µg

Lane 3:

TCMK-1 whole cell lysate at 50 µg

Lane 4:

BW5147.3 whole cell lysate at 50 µg

Lane 5:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR060F

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human BCKDHA phospho S292. The exact immunogen used to generate this antibody is proprietary information.

P12694

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99.81% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.

See full target information BCKDHA phospho S292

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 108:961-974 PubMed37139700

2023

High doses of rosuvastatin induce impaired branched-chain amino acid catabolism and lead to insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Bai,Xingzhen Long,Fang Song,Baolin Chen,Changcheng Sheng,Cailin Tang,Li Li,Jiaxing Zhang,Rui Zhang,Jiquan Zhang,Jiali Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCKDHA (phospho S292) antibody [BLR060F]

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise