AB200577

Anti-BCKDHA (phospho S293) antibody

Name: Anti-BCKDHA (phospho S293) antibody. Rabbit polyclonal (ab200577) | Abcam
Brand: Abcam Limited
SKU: AB200577
Price: 450 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(28 Publications)

Rabbit Polyclonal BCKDHA phospho S293 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human BCKDHA phospho S293.

View Alternative Names

Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA

2 Images

Supplier Data

Western blot - Anti-BCKDHA (phospho S293) antibody (AB200577)

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-BCKDHA (phospho S293) antibody (ab200577) at 1/25000 dilution

Lane 1:

Hep-G2 Whole cell lysate at 10 µg

Lane 2:

HeLa Whole cell lysate at 10 µg

Lane 3:

LNCaP Whole cell lysate at 10 µg

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-BCKDHA (phospho S293) antibody (AB200577)

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-BCKDHA (phospho S293) antibody (ab200577) at 1/25000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 10 µg

Lane 2:

CT26 Whole cell lysate at 10 µg

Lane 3:

CH27 Whole cell lysate at 10 µg

Lane 4:

TCMK-1 Whole cell lysate at 10 µg

Lane 5:

BW5147.3 Whole cell lysate at 10 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human BCKDHA phospho S293. The exact immunogen used to generate this antibody is proprietary information.

P12694

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.

See full target information BCKDHA phospho S293

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2416590 PubMed40298908

2025

Nuclear-Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Liu,Jiaqian Feng,Tingting Pan,Pinggen Zhang,Ling Ye,Zetan Jiang,Zilong Zhou,Qiankun Mao,Jian Li,Xinyi Yang,Ping Gao,De Huang,Huafeng Zhang

Journal of neuroinflammation 21:327 PubMed39716292

2024

Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Guanjin Shi,Yanyan Ge,Shanshan Huang,Ningning Cui,Le Tan,Rui Liu,Xuefeng Yang

Heliyon 10:e33663 PubMed39170503

2024

FYN-mediated phosphorylation of BCKDK at Y151 promotes GBM proliferation by increasing the oncogenic metabolite N-acetyl-L-alanine.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zou,Wei Wang,Wenda Huang,Xiaofang Ni,Wensheng Li,Yue Cheng,Qin Tian,Lin Liu,Feng Zhu,Qiuhong Duan

Acta diabetologica 62:227-240 PubMed39150511

2024

Accumulation of branched-chain amino acids deteriorates the neuroinflammatory response of Müller cells in diabetic retinopathy via leucine/Sestrin2-mediated sensing of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Gong,Jingyi Wang,Dawei Luo,Yupeng Xu,Rulin Zhang,Xin Li,Zihan Yin,Junwei Fang,Haiyan Wang

Nucleic acids research 52:6629-6646 PubMed38567728

2024

Mitolnc controls cardiac BCAA metabolism and heart hypertrophy by allosteric activation of BCKDH.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Weiss,Sara Hettrich,Theresa Hofmann,Salma Hachim,Stefan Günther,Thomas Braun,Thomas Boettger

Molecular cell 83:3904-3920.e7 PubMed37879334

2023

Lactate activates the mitochondrial electron transport chain independently of its metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Cai,Charles P Ng,Olivia Jones,Tak Shun Fung,Keun Woo Ryu,Dayi Li,Craig B Thompson

Cell death & disease 14:634 PubMed37752100

2023

PPM1K mediates metabolic disorder of branched-chain amino acid and regulates cerebral ischemia-reperfusion injury by activating ferroptosis in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Lili Zhao,Ye Li,Meijuan Dang,Jialiang Lu,Ziwei Lu,Qiao Huang,Yang Yang,Yuxuan Feng,Xiaoya Wang,Yating Jian,Heying Wang,Yingying Guo,Lei Zhang,Yu Jiang,Songhua Fan,Shengxi Wu,Hong Fan,Fang Kuang,Guilian Zhang

Cell reports 42:112641 PubMed37310861

2023

Branched-chain keto acids inhibit mitochondrial pyruvate carrier and suppress gluconeogenesis in hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kiyoto Nishi,Akira Yoshii,Lauren Abell,Bo Zhou,Ricardo Frausto,Julia Ritterhoff,Timothy S McMillen,Ian Sweet,Yibin Wang,Chen Gao,Rong Tian

PPAR research 2023:4779199 PubMed37325367

2023

PPAR/ Agonism with GW501516 Increases Myotube PGC-1 Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline N Rivera,Jason S Hinkle,Rachel M Watne,Trent C Macgowan,Andrew J Wommack,Roger A Vaughan

Nutrients 15: PubMed37049555

2023

Reduction of Plasma BCAAs following Roux-en-Y Gastric Bypass Surgery Is Primarily Mediated by FGF21.

Applications

Unspecified application

Species

Unspecified reactive species

Harsh Shah,Alyssa Kramer,Caitlyn A Mullins,Marie Mattern,Ritchel B Gannaban,R Leigh Townsend,Shawn R Campagna,Christopher D Morrison,Hans-Rudolf Berthoud,Andrew C Shin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCKDHA (phospho S293) antibody

Western blot - Anti-BCKDHA (phospho S293) antibody (AB200577)

Western blot - Anti-BCKDHA (phospho S293) antibody (AB200577)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (28)

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