AB151297

Anti-BCKDK antibody

Name: Anti-BCKDK antibody (ab151297) | Abcam
Brand: Abcam Limited
SKU: AB151297
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(4 Publications)

Rabbit Polyclonal BCKDK antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BCKDK aa 50-400.

View Alternative Names

Branched-chain alpha-ketoacid dehydrogenase kinase, BCKDH kinase, BCKDHKIN, BDK, BCKDK

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDK antibody (AB151297)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDK antibody (AB151297)

Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling BCKDK with ab151297 antibody at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-BCKDK antibody (AB151297)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BCKDK antibody (AB151297)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling BCKDK protein at mitochondria using ab151297 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-BCKDK antibody (AB151297)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-BCKDK antibody (ab151297) at 1/1000 dilution

Lane 1:

MCF-7 whole cell extracts at 30 µg

Lane 2:

MCF-7 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-BCKDK antibody (AB151297)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-BCKDK antibody (ab151297) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-BCKDK antibody (AB151297)

Samples were separated by 10 % SDS. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-BCKDK antibody (ab151297) at 1/10000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

true

Supplier Data

Western blot - Anti-BCKDK antibody (AB151297)

All lanes:

Western blot - Anti-BCKDK antibody (ab151297) at 1/10000 dilution

All lanes:

Rat kidney extract at 50 µg

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-BCKDK antibody (AB151297)

All lanes:

Western blot - Anti-BCKDK antibody (ab151297) at 1/10000 dilution

All lanes:

Rat brain extract at 50 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human BCKDK aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

O14874

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets : mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle (PubMed : 24449431, PubMed : 29779826, PubMed : 37558654). Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism : E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex (PubMed : 29779826, PubMed : 37558654). Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively (PubMed : 29779826). Recognizes phosphosites having SxxE/D canonical motif (PubMed : 29779826).

See full target information BCKDK

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta diabetologica 62:227-240 PubMed39150511

2024

Accumulation of branched-chain amino acids deteriorates the neuroinflammatory response of Müller cells in diabetic retinopathy via leucine/Sestrin2-mediated sensing of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Gong,Jingyi Wang,Dawei Luo,Yupeng Xu,Rulin Zhang,Xin Li,Zihan Yin,Junwei Fang,Haiyan Wang

iScience 24:103342 PubMed34805797

2021

spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Naofumi Yoshida,Tomoya Yamashita,Tatsunori Osone,Tetsuya Hosooka,Masakazu Shinohara,Seiichi Kitahama,Kengo Sasaki,Daisuke Sasaki,Takeshi Yoneshiro,Tomohiro Suzuki,Takuo Emoto,Yoshihiro Saito,Genki Ozawa,Yushi Hirota,Yasuyuki Kitaura,Yoshiharu Shimomura,Yuko Okamatsu-Ogura,Masayuki Saito,Akihiko Kondo,Shingo Kajimura,Takeshi Inagaki,Wataru Ogawa,Takuji Yamada,Ken-Ichi Hirata

Cell metabolism 29:1151-1165.e6 PubMed30661928

2019

Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Russell E Ericksen,Siew Lan Lim,Eoin McDonnell,Wai Ho Shuen,Maya Vadiveloo,Phillip J White,Zhaobing Ding,Royston Kwok,Philip Lee,George K Radda,Han Chong Toh,Matthew D Hirschey,Weiping Han

Oncology reports 39:71-80 PubMed29115564

2017

miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2.

Applications

Unspecified application

Species

Unspecified reactive species

Baoping Lang,Song Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BCKDK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDK antibody (AB151297)

Immunocytochemistry/ Immunofluorescence - Anti-BCKDK antibody (AB151297)

Western blot - Anti-BCKDK antibody (AB151297)

Western blot - Anti-BCKDK antibody (AB151297)

Western blot - Anti-BCKDK antibody (AB151297)

Western blot - Anti-BCKDK antibody (AB151297)

Western blot - Anti-BCKDK antibody (AB151297)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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