Mouse Monoclonal Bcl-2 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BCL2 aa 1-100.
Images
![Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (AB212800), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-100-d5-bsa-and-azide-free-ab212800--western-blot-img85173.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (AB212800)")
![Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (AB212800), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-100-d5-bsa-and-azide-free-ab212800--flow-cytometry-img57534.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (AB212800)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (AB212800), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-100-d5-bsa-and-azide-free-ab212800--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58633.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (AB212800)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
| Cow | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes (in 0.1ml volume) |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes (in 0.1ml volume) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes (in 0.1ml volume) |
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes (for 30 minutes at RT) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes (for 30 minutes at RT) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes (for 30 minutes at RT) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Mouse Monoclonal Bcl-2 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BCL2 aa 1-100.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
- Carrier free
Yes
- Clone number
100/D5
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
ab212800 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-100-d5-bsa-and-azide-free-ab212800--western-blot-img85173.jpg "Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)")
Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
All lanes: Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800) at 1 µg/mL
All lanes: Human skin lysate
Predicted band size: 26 kDa
![Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-100-d5-bsa-and-azide-free-ab212800--flow-cytometry-img57534.jpg "Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)")
Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
Flow cytometric analysis of Jurkat cells labeling Bcl-2 uwith ab212800 at 1μg/106 cells (red), compared with an isotype control (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-100-d5-bsa-and-azide-free-ab212800--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58633.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-Hodgkin's Lymphoma tissue labeling Bcl-2 with ab212800 at 1 μg/ml.
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