Anti-Bcl-2 antibody (ab194583) is a rabbit polyclonal antibody detecting Bcl-2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 140 publications
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Effects of Nutraceuticals on Cisplatin-Induced Cytotoxicity in HEI-OC1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorenzo Guidotti,Elena Tomassi,Silvia Marracci,Michele Lai,Dominga Lapi,Rossana Pesi,Laura Pucci,Ettore Novellino,Elisabetta Albi,Mercedes Garcia-GilPubMed 38139245
- Polish journal of pathology : official journal of the Polish Society of Pathologists 74:182-1932023S100A16 cooperates with DEPDC1 to promote the progression and angiogenesis of nephroblastoma through PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeng Geng,Yongtao Xu,Qingfang Li,Qinghao Li,Lili Yuan,Mengyao Dong,Ming MingPubMed 37955537
- Aging 15:12264-122742023Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhipeng Tang,Xiuqin Li,Nan Yin,Ming Zhao,Qingchuan Hu,Peiyuan LvPubMed 37934569
- Antioxidants (Basel, Switzerland) 12:2023DDAH1 Protects against Cardiotoxin-Induced Muscle Injury and Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Feng,Bingqing Cui,Li Fang,Ting Lan,Kai Luo,Xin Xu,Zhongbing LuPubMed 37760057
- Frontiers in medicine 10:12309412023Restored retinal physiology after administration of niacin with citicoline in a mouse model of hypertensive glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Melecchi,Rosario Amato,Massimo Dal Monte,Dario Rusciano,Paola Bagnoli,Maurizio CammalleriPubMed 37731716
- Phytotherapy research : PTR 37:4740-47542023Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Li,Guoli Wang,Xiaolin Zhou,Huijie Zhao,Xiaojie Chen,Qixiao Cui,Minjing Li,Xihang Gao,Xiaoyu Wei,Lei Ye,Defang Li,Pan HongPubMed 37559472
- World journal of surgical oncology 21:2052023Lung cancer-derived exosomal miR-132-3p contributed to interstitial lung disease development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSufang Fang,Ting Wang,Ling Weng,Ximei Han,Rongshan Zheng,Hongying ZhangPubMed 37454094
- Iranian journal of pharmaceutical research : IJPR 22:e1324962023NBP Cytoprotective Effects Promoting Neuronal Differentiation in BMSCs by Inhibiting the p65/Hes1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEryi Zhao,Peijian Huang,Zhongyan Zhao,Shixiong Huang,Shijun Hu,Ling Xie,Jie Lin,Daimei WangPubMed 38116559
- European journal of histochemistry : EJH 67:2023LncRNA gadd7 promotes mitochondrial membrane potential decrease and apoptosis of alveolar type II epithelial cells by positively regulating MFN1 in an model of hyperoxia-induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoyue Liu,Cunzhi Yin,Mingjiang Qian,Xuan Xiao,Hang Wu,Fujian FuPubMed 37254890
- Immunity, inflammation and disease 11:e8242023CircSmox knockdown alleviates PC12 cell apoptosis and inflammation in spinal cord injury by miR-340-5p/Smurf1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyin Han,Zufang Mou,Yulong Jing,Rong Jiang,Tao SunPubMed 37102659
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BCL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
Consider this alternative
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Tested | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 - 1/100 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info 1/50 - 1/100 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/50 - 1/100 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 - 1/100 | Notes - |
Species Mouse | Dilution info 1/50 - 1/100 | Notes - |
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Anti-Bcl-2 antibody (ab194583) is a rabbit polyclonal antibody detecting Bcl-2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 140 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BCL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Bcl-2 antibody (ab194583) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Bcl-2?
Anti-Bcl-2 (ab194583) specifically detects a band for Bcl-2 (UniProt: P10415) at a molecular weight of 26kDa.
Trusted by the scientific community
Anti-Bcl-2 (ab194583) was first used in a scientific publication in 2014 and has been cited over 140 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-Bcl-2 antibody (ab194583)
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.All lanes: Western blot - Anti-Bcl-2 antibody (ab194583) at 1/500 dilution
Lane 1: 239T cell lysate at 25 µg
Lane 2: 239T Bcl-2 knock-out cell lysate at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDa
Immunoprecipitation - Anti-Bcl-2 antibody (ab194583)
Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (ab194583). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (ab194583) at a dilition of 1:1000.
All lanes: Immunoprecipitation - Anti-Bcl-2 antibody (ab194583)
Predicted band size: 26 kDa
Western blot - Anti-Bcl-2 antibody (ab194583)
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic KitExposure time: 30 seconds
All lanes: Western blot - Anti-Bcl-2 antibody (ab194583) at 1/500 dilution
Lane 1: Mouse lung cell extract at 25 µg
Lane 2: Mouse spleen cell extract at 25 µg
Lane 3: Rat spleen cell extract at 25 µg
Lane 4: Rat ovary cell extract at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab194583)
Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab194583)
Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab194583)
Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (ab194583) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com