AB194583

Anti-Bcl-2 antibody

Name: Anti-Bcl-2 antibody (ab194583) | Abcam
Brand: Abcam Limited
SKU: AB194583
Price: 575 USD
Availability: InStock

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(228 Publications)

Anti-Bcl-2 antibody (ab194583) is a rabbit polyclonal antibody detecting Bcl-2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 140 publications

View Alternative Names

Apoptosis regulator Bcl-2, BCL2

6 Images

Supplier Data

Western blot - Anti-Bcl-2 antibody (AB194583)

Blocking buffer : 3% nonfat dry milk in TBST.
Detection : ECL Basic Kit (RM00020).
Exposure time : 30s.

All lanes:

Western blot - Anti-Bcl-2 antibody (ab194583) at 1/500 dilution

Lane 1:

239T cell lysate at 25 µg

Lane 2:

239T Bcl-2 knock-out cell lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 26 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (AB194583)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (AB194583)

Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1 : 100 (40x lens). Blue : DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (AB194583)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (AB194583)

Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (ab194583) at dilution of 1 : 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Bcl-2 antibody (AB194583)

Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (ab194583). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (ab194583) at a dilition of 1 : 1000.

All lanes:

Immunoprecipitation - Anti-Bcl-2 antibody (ab194583)

Predicted band size: 26 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (AB194583)

Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1 : 100 (40x lens). Blue : DAPI for nuclear staining.

Supplier Data

Western blot - Anti-Bcl-2 antibody (AB194583)

Blocking buffer : 3% nonfat dry milk in TBST.
Detection : ECL Basic Kit

Exposure time : 30 seconds

All lanes:

Western blot - Anti-Bcl-2 antibody (ab194583) at 1/500 dilution

Lane 1:

Mouse lung cell extract at 25 µg

Lane 2:

Mouse spleen cell extract at 25 µg

Lane 3:

Rat spleen cell extract at 25 µg

Lane 4:

Rat ovary cell extract at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human BCL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P10415

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Bcl-2 antibody (ab194583) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Bcl-2?
Anti-Bcl-2 (ab194583) specifically detects a band for Bcl-2 (UniProt: P10415) at a molecular weight of 26kDa.

Trusted by the scientific community
Anti-Bcl-2 (ab194583) was first used in a scientific publication in 2014 and has been cited over 140 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.

Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).

See full target information BCL2

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Publications (228)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed41062301

2025

KLF5 Alleviates Excessive Autophagy-Induced Podocyte Injury by Enhancing ITCH-Mediated Ubiquitination of ACTR2.

Applications

Unspecified application

Species

Unspecified reactive species

Xianchu Lin,Huirong Lin,Man Luo,Jun Han,Yan Hu,Shilong Xiang,Zejun Fang

Annals of medicine and surgery (2012) 87:5512-5521 PubMed40901091

2025

Electroacupuncture therapy improves cognitive dysfunction after ischemic stroke in Sprague-Dawley rats by adjusting the lncRNA-MEG3/miR-4640-3p axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Shiqing Gao,Ling Lin,Yongbing Zheng

Investigative ophthalmology & visual science 66:29 PubMed40793857

2025

Inhibiting The uPA/uPAR Pathway Affords Photoreceptor Resilience and Preserves Retinal Function in a Mouse Model of Retinitis Pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Rosario Amato,Alessio Canovai,Alberto Melecchi,Maria De Fenza,Linda Leone,Vincenzo Pavone,Daniele D'Alonzo,Maurizio Cammalleri,Massimo Dal Monte

iScience 28:112834 PubMed40687840

2025

Reactive oxygen species-responsive hydrogel loaded with melatonin as a minimally invasive treatment for myocardial I/R injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hechen Shen,Xu Bao,Bingcai Qi,Xiaoyu Liang,Yun Chang,Xiaomin Hu,Wenqing Gao,Di Sun

Drug design, development and therapy 19:5871-5898 PubMed40661830

2025

Protects Against Steroid-Induced Osteonecrosis of the Femoral Head Through Inhibiting Inflammatory Responses and Apoptosis via Network Pharmacology and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yuhang Fan,Mengjiao Li,Zheming Song,Pengcheng Wang,Fei Xie,Yuqi Miao,Yifan Wang,Peng Zhang,Qiang Zhan

Journal of microbiology and biotechnology 35:e2412067 PubMed40582762

2025

Effects of Acute Abdomen III on Sepsis-Induced Intestinal Damage in a Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Jingjing Hu,Fangyu Hu,Yuexuan Chen,Ming Fang,Yuanling Ye

Food science & nutrition 13:e70464 PubMed40535916

2025

Ginsenoside Rh1 Alleviates Allergic Rhinitis by Mediating Mitochondrial Autophagy via Activation of the AMPK/ULK1/FUNDC1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangang Wang,Yalin Zhang,Jingmei Chai,Jianing Yang,Longzhu Dai,Yi Yang,Yulian Zhang,Yongde Jin,Chongyang Wang,Guanghai Yan

IBRO neuroscience reports 18:759-770 PubMed40502966

2025

Alterations in apoptotic pathways and expression of miR-134, miR-181, and miR-497 induced by Wharton's jelly-derived mesenchymal stem cells in a rat model of ischemic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tahereh Alizamir,Ali Fathi Jouzdani,Fatemeh Attari,Leila Arab,Zeinab Ashaari,Alireza Komaki,Gholamreza Hassanzadeh

Translational lung cancer research 14:1351-1370 PubMed40386722

2025

was stabilized through N-methyladenosine methylation and exerted its anticancer effects in lung squamous cell carcinoma by inhibiting SIRT1 activity via CCAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Yan,Wingshing Wong,Jinsong Lei,Dachuan Liang,Jie Yang,Li Gong,Rossana Berardi,Shuqin Dai,Junye Wang

The Journal of biological chemistry 301:110242 PubMed40381695

2025

Machine learning-based multimodal radiomics and transcriptomics models for predicting radiotherapy sensitivity and prognosis in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyu Ye,Hao Zhang,Zhou Chi,Zhina Xu,Yujie Cai,Yajing Xu,Xiangmin Tong

View all publications

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