Anti-Bcl-2 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (AB59348)

ab59348, at a 1/50 dilution, staining human Bcl-2 in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (AB59348)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Bcl-2 with ab59348 in the absence (left image) and presence of the immunizing peptide (right image).

• WB

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Western blot - Anti-Bcl-2 antibody (AB59348)

Blocking buffer : 5% (w/v) non-fat dry milk in TBST.

Primary antibody dilution buffer : 5%（w/v）non-fat dried milk，0.1%（v/v）, Tween-20 in TBST.

Secondary antibody dilution buffer : 5%（w/v）non-fat dried milk，0.1%（v/v），Tween-20 in TBST.

12% SDS gel. Nitrocellulose membrane.

Blocking : Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.

Primary antibody incubation : diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.

Secondary antibody incubation : diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.

ECL development.

All lanes:

Western blot - Anti-Bcl-2 antibody (ab59348) at 1/500 dilution

Lane 1:

Extracts from K562 cells at 40 µg with no immunizing peptide

Lane 2:

Extracts from K562 cells at 40 µg with immunizing peptide

Predicted band size: 26 kDa

Observed band size: 26 kDa

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