Rabbit Polyclonal Bcl-2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 645 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2.
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Publications
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- Acta cirurgica brasileira 38:e3844232023Effects of daidzein on rat ovary against ischemia-reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeysel Toprak,Senem Alkan Akalın,Ece Öcal,Yunus Çavuş,Engin DeveciPubMed 37909594
- Experimental and therapeutic medicine 26:5062023Natural product procyanidin B1 as an antitumor drug for effective therapy of colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongdong Lei,Xiaorong Deng,Zhenghong Zhang,Jiluan ChenPubMed 37822589
- Drug design, development and therapy 17:2209-22222023Elucidating the Mechanism of Agrimonolide in Treating Colon Cancer Based on Network Pharmacology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yu,Yun GaiPubMed 37533972
- Cell death discovery 9:2472023The inhibition of FKBP5 protects β-cell survival under inflammation stress via AKT/FOXO1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Liu,Rui Li,Jinglin Cao,Xinyao Song,Wenmiao Ma,Tengli Liu,Le Wang,Jiaqi Zou,Boya Zhang,Zewen Liu,Rui Liang,Rongxiu Zheng,Shusen WangPubMed 37452039
- Iranian journal of basic medical sciences 26:785-7902023Therapeutic effects of diosgenin on alveolar bone loss and apoptosis in diabetic rats with experimental periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlper Kızıldağa,Aysan Lektemür Alpanb,Melih Özdedec,Tuğba Aydınd,Özlem ÖzmenePubMed 37396943
- Cell death discovery 9:2182023Tim-3 protects against cisplatin nephrotoxicity by inhibiting NF-κB-mediated inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeiyao Li,Xuemiao Li,Wenbin Wu,Mengjia Hou,Guanyi Yin,Zhonghang Wang,Ziyu Du,Yuanfang Ma,Qiang Lou,Yinxiang WeiPubMed 37393392
- Immunity, inflammation and disease 11:e9042023Epimedium sagittatum Maxim ameliorates adriamycin-induced nephropathy by restraining inflammation and apoptosis via the PI3K/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu Wang,Mengnan Zeng,Beibei Zhang,Qinqin Zhang,Shuangshuang Xie,Yingbo Hu,Ruyi Fan,Mengya Wang,Xiao Yu,Yuhan Zhang,Xiaoke Zheng,Weisheng FengPubMed 37382268
- Life science alliance 6:2023MiR-93-5p inhibits retinal neurons apoptosis by regulating PDCD4 in acute ocular hypertension model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Tan,Wenjia Shi,Yun Zhang,Can Liu,Tu Hu,Dan Chen,Jufang HuangPubMed 37308277
- International journal of molecular sciences 24:2023Synaptamide Ameliorates Hippocampal Neurodegeneration and Glial Activation in Mice with Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Tyrtyshnaia,Olga Manzhulo,Igor ManzhuloPubMed 37373162
- Heliyon 9:e159452023Protective effects of cell permeable Tat-PIM2 protein on oxidative stress induced dopaminergic neuronal cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jea Shin,Won Sik Eum,Gi Soo Youn,Jung Hwan Park,Hyeon Ji Yeo,Eun Ji Yeo,Hyun Jung Kwon,Eun Jeong Sohn,Lee Re Lee,Na Yeon Kim,Su Yeon Kwon,Su Min Kim,Hyo Young Jung,Duk-Soo Kim,Sung-Woo Cho,Oh-Shin Kwon,Dae Won Kim,Soo Young ChoiPubMed 37223703
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human BCL2. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Positive control: K562 cell lysate. Please see WB protocol details in the image legend. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Associated Products
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Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Rabbit Polyclonal Bcl-2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 645 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human BCL2. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Affinity purified from rabbit antiserum by affinity chromatography, usingepitope specific immunogen
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Store at -20°C for year
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Bcl-2 antibody (ab59348)
Blocking buffer: 5% (w/v) non-fat dry milk in TBST.
Primary antibody dilution buffer: 5%（w/v）non-fat dried milk，0.1%（v/v）, Tween-20 in TBST.
Secondary antibody dilution buffer: 5%（w/v）non-fat dried milk，0.1%（v/v），Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
All lanes: Western blot - Anti-Bcl-2 antibody (ab59348) at 1/500 dilution
All lanes: Extracts from K562 cells at 40 µg
Predicted band size: 26 kDa
Observed band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab59348)
ab59348, at a 1/50 dilution, staining human Bcl-2 in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab59348)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Bcl-2 with ab59348 in the absence (left image) and presence of the immunizing peptide (right image).
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