Mouse Monoclonal Bcl-2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2 aa 1-100.
Images
![Western blot - Anti-Bcl-2 antibody [Bcl2/100] (AB117115), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-100-ab117115--western-blot-img100517.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [Bcl2/100] (AB117115)")
![Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (AB117115), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-100-ab117115--flow-cytometry-intracellular-img428825.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (AB117115)")
![Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (AB117115), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-100-ab117115--flow-cytometry-intracellular-img428824.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (AB117115)")
Publications
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- International journal of molecular sciences 24:2023Discovery of Salidroside as a Novel Non-Coding RNA Modulator to Delay Cellular Senescence and Promote BK-Dependent Apoptosis in Cerebrovascular Smooth Muscle Cells of Simulated Microgravity Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiling Ge,Bin Zhang,Jibo Song,Qinglin Cao,Yingrui Bu,Peijie Li,Yungang Bai,Changbin Yang,Manjiang XiePubMed 37833978
- Journal of veterinary research 67:307-3142023Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Hye Lee,Sang-Hwan KimPubMed 38143820
- Cancer gene therapy 30:1007-10172023E2F1-mediated KDM4A-AS1 up-regulation promotes EMT of hepatocellular carcinoma cells by recruiting ILF3 to stabilize AURKA mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao-Ming Shen,Di Zhang,Ping Xiao,Bin Qu,Yi-Fan SunPubMed 36973424
- Molecules (Basel, Switzerland) 27:2022Induction of Apoptosis via Inactivating PI3K/AKT Pathway in Colorectal Cancer Cells Using Aged Chinese Hakka Stir-Fried Green Tea Extract.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyue Zhang,Haiying Huang,Shili Sun,Dongli Li,Lingli Sun,Qiuhua Li,Ruohong Chen,Xingfei Lai,Zhenbiao Zhang,Xi Zheng,Wing-Leung Wong,Shuai WenPubMed 36500365
- Nutrients 14:2022Shibi Tea () and Camellianin A Alleviate CCl-Induced Liver Injury in C57BL-6J Mice by Attenuation of Oxidative Stress, Inflammation, and Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuohong Chen,Yingyi Lian,Shuai Wen,Qiuhua Li,Lingli Sun,Xingfei Lai,Zhenbiao Zhang,Junquan Zhu,Linsong Tang,Ji Xuan,Erdong Yuan,Shili SunPubMed 35893891
- Journal of biochemical and molecular toxicology 36:e231182022Negative regulation of miR-1288-3p/KRT4 axis through a circular RNA in oral cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Wang,Xin Ma,Jie Yu,Ying LouPubMed 35707935
- Molecular medicine reports 25:2021CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinjin Li,Yiren Hu,Dake Chen,Jianlong Zhu,Wenshuo Bao,Xiaomin Xu,Heyi Chen,Wu Chen,Rui FengPubMed 34791506
- Cancer cell international 21:4852021Engineered exosomes loaded with miR-449a selectively inhibit the growth of homologous non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Zhou,Mingming Xu,Zhipeng Wang,Mingjun YangPubMed 34521413
- American journal of translational research 13:8728-87412021miR-590-5p affects chondrocyte proliferation, apoptosis, and inflammation by targeting FGF18 in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Jiang,Xixi Dou,Shuaiguang Li,Qingwen Jia,Peixue Ling,Hongyu Liu,Qiuju Han,Shui SunPubMed 34539990
- Acta veterinaria Hungarica 69:169-1742021Galectin-3 immunolabelling correlates with BCL2 expression in canine cutaneous mast cell tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThiago Henrique M Vargas,Camila N Barra,Lidia H Pulz,Greice C Huete,Karine G Cadrobbi,Adriana Tomoko Nishiya,Silvia Regina Kleeb,José Guilherme Xavier,José Luiz Catão-Dias,Ricardo F StrefezziPubMed 34111022
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
Reactivity data
Select an application| WB | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Not recommended | Not recommended |
| Cat | Not recommended | Predicted |
| Cow | Not recommended | Predicted |
| Pig | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Cat, Pig | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Pig | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Mouse Monoclonal Bcl-2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2 aa 1-100.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P10415
- Clone number
Bcl2/100
- Purification technique
Affinity purification Protein A
- Concentration
- Purification notes
ab117115 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE).
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-100-ab117115--western-blot-img100517.jpg "Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)")
Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: BCL2 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: THP-1 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab117115 observed at 26 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab117115 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. ab117115 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-100-ab117115--flow-cytometry-intracellular-img428825.jpg "Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)")
Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
Flow cytometric analysis of Human peripheral whole blood labeling Bcl-2 with ab117115 at 1 µg/mL.
![Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-100-ab117115--flow-cytometry-intracellular-img428824.jpg "Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)")
Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
Flow cytometric analysis of Human peripheral whole blood labeling Bcl-2 with ab117115 at 1 µg/mL, showing the separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
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