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AB238041

Anti-Bcl-2 antibody [BCL2/2210R]

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(4 Publications)

Rabbit Recombinant Monoclonal Bcl-2 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BCL2.

View Alternative Names

Apoptosis regulator Bcl-2, BCL2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] (AB238041)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] (AB238041)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Bcl-2 using ab238041 at 1 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] (AB238041)
  • Protein Array

Unknown

Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] (AB238041)

ab238041 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot - Anti-Bcl-2 antibody [BCL2/2210R] (AB238041)
  • WB

Supplier Data

Western blot - Anti-Bcl-2 antibody [BCL2/2210R] (AB238041)

All lanes:

Western blot - Anti-Bcl-2 antibody [BCL2/2210R] (ab238041) at 1 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BCL2/2210R

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Protein Array

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BCL2.

P10415

Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).
See full target information BCL2

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The journal of gene medicine 25:e3507 PubMed36999202

2023

Circ_0073228 serves as a competitive endogenous ribonucleic acid to facilitate proliferation and inhibit apoptosis of hepatocellular carcinoma cells by the miR-139-5p/deoxyribonuclease II axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Bai,Zujiang Yu,Qinggang Li,Xiaobo Hu,Xiaorui Liu,Fulei Li,Shasha Li,Yanling Chen

Molecular and cellular biology 41:e0006521 PubMed34001648

2021

CEBPA-AS1 Knockdown Alleviates Oxygen-Glucose Deprivation/Reperfusion-Induced Neuron Cell Damage by the MicroRNA 24-3p/BOK Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangfu Di,Xinjie Yang,Feng Cheng,Hua Liu,Min Xu

Open life sciences 16:495-510 PubMed34056113

2021

Mechanisms of circular RNA circ_0066147 on pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Zhang Zhang

Artificial cells, nanomedicine, and biotechnology 47:2265-2273 PubMed31184221

2019

Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Wang,Xinxia Zhang,Yutong Han,Qiuli Wang,Yanfan Ren,Baojun Wang,Junxi Hu
View all publications

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