Rabbit Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BCL2.
Images
![Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (AB237891), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-2210r-bsa-and-azide-free-ab237891--protein-array-img141834.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (AB237891)")
![Western blot - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (AB237891), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-2210r-bsa-and-azide-free-ab237891--western-blot-img8762.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (AB237891)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (AB237891), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-2210r-bsa-and-azide-free-ab237891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120236.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (AB237891)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human BCL2. Database link P10415
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Boil tissue sections in 1mM EDTA Buffer, pH 8.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Rabbit Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BCL2.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human BCL2. Database link P10415
- Carrier free
Yes
- Clone number
BCL2/2210R
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-2210r-bsa-and-azide-free-ab237891--protein-array-img141834.jpg "Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891)")
Protein Array - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891)
This data was produced with Anti-Bcl-2 antibody [BCL2/2210R] ab238041, the same antibody in a different formulation with BSA and Azide.
Anti-Bcl-2 antibody [BCL2/2210R] ab238041 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Western blot - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-2210r-bsa-and-azide-free-ab237891--western-blot-img8762.jpg "Western blot - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891)")
Western blot - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891)
All lanes: Western blot - Anti-Bcl-2 antibody [BCL2/2210R] (Anti-Bcl-2 antibody [BCL2/2210R] ab238041) at 1 µg/mL
All lanes: MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-bcl2-2210r-bsa-and-azide-free-ab237891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120236.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/2210R] - BSA and Azide free (ab237891)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Bcl-2 using ab237891 at 1 μg/ml in immunohistochemical analysis.
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