Rabbit Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 98 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70657.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62746.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002)")
![Immunoprecipitation - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunoprecipitation-img64148.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002)")
![Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--western-blot-img5652.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81067.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (AB185002)")
Publications
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- Aging 15:12120-121352023USP14 predicts poorer survival outcomes and promotes tumor progression in endometrial carcinoma by activating NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojin Gong,Li Jia,Lili Zhou,Tongxiu HuPubMed 37917013
- Inflammation 47:421-4372023lncRNA HOTAIRM1 Activated by HOXA4 Drives HUVEC Proliferation Through Direct Interaction with Protein Partner HSPA5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhou,Qiang Wu,Xiangshu Long,Youfu He,Jing HuangPubMed 37898994
- Gut and liver 16:861-8742022Circ_0081143 Contributes to Gastric Cancer Malignant Development and Doxorubicin Resistance by Elevating the Expression of YES1 by Targeting mziR-129-2-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenting Ou,Lin Lin,Rihong Chen,Qingwen Xu,Caijin ZhouPubMed 35686503
- Applied biochemistry and biotechnology 194:3819-38322022LncRNA LINC00665 Promotes Ovarian Cancer Cell Proliferation and Inhibits Apoptosis via Targeting miR-181a-5p/FHDC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuli Wang,Yingchun Wang,Jin Lu,Jinhua WangPubMed 35524876
- Cell death discovery 8:812022The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis.Applications:WBReactive species:HumanYu Yan,Yu Nie,Chun Peng,Fuchen Xing,Saiguang Ji,Hong Liu,Chuandong ZhuPubMed 35210429
- Frontiers in oncology 11:8032782022HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEster Badami,Claudia Carcione,Cinzia Maria Chinnici,Rosaria Tinnirello,Pier Giulio Conaldi,Gioacchin IannoloPubMed 35127513
- Annals of translational medicine 9:17222022Aloe-emodin inhibits the proliferation, migration, and invasion of melanoma cells via inactivation of the Wnt/beta-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaotao Du,Pan Shen,Ranjing Tan,Dengyan Wu,Shenghao TuPubMed 35071416
- Frontiers in pharmacology 12:7237842022Ginsenoside Rb1 Attenuates Triptolide-Induced Cytotoxicity in HL-7702 Cells the Activation of Keap1/Nrf2/ARE Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHulinyue Peng,Longtai You,Chunjing Yang,Kaixin Wang,Manting Liu,Dongge Yin,Yuchen Xu,Xiaoxv Dong,Xingbin Yin,Jian NiPubMed 35046796
- Cell death discovery 8:142022Knockdown of NRMT enhances sensitivity of retinoblastoma cells to cisplatin through upregulation of the CENPA/Myc/Bcl2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongrui Li,Lan Zhang,Dongrui Liu,Zhanghui Yang,Di Xuan,Yi ZhangPubMed 35013138
- Experimental and therapeutic medicine 23:712021MicroRNA-208a-3p participates in coronary heart disease by regulating the growth of hVSMCs by targeting BTG1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Wang,Caiyun YanPubMed 34934442
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Please check the parent abID, Anti-Bcl-2 antibody [E17] ab32124, for a recommended dilution. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Rabbit Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 98 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
E17
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognises Bcl-2. It does not cross-react with other Bcl-2 family members. Bcl-2 has two isoforms, one is around 26kDa and the other is around 20kDa (PMID: 26009263, PMID: 10400666, PMID: 32377726).
- Dissociation constant
3 x 10-11 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab185002 is the carrier-free version of Anti-Bcl-2 antibody [E17] ab32124.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70657.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Image from Némati F et al. PLoS One. 2014;9(1):e80836. Fig 2.; doi: 10.1371/journal.pone.0080836.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Bcl-2 expression determined by immunohistochemical analyses of the 4 human UM xenografts (between 3 to 5 tumors have been studied per condition).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62746.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Image from Szyszko EA et al. Arthritis Res Ther. 2011 Jan 7;13(1):R2. Fig 5.; doi:10.1186/ar3220; 7 January 2011 Arthritis Research & Therapy 2011 13:R2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Immunohistochemical analysis of Human salivary glands taken from patients with primary Sjögren's syndrome, staining Bcl-2 with unpurified Anti-Bcl-2 antibody [E17] ab32124.
Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked using 2% BSA, 10% normal serum and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100) for one hour at room temperature. An Alexa Fluor® 594-conjugated anti-rabbit IgG was used as the secondary antibody.
N.B. Panels B and D are higher magnifications of panels A and C, respectively.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
![Immunoprecipitation - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunoprecipitation-img64148.jpg "Immunoprecipitation - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Immunoprecipitation - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Anti-Bcl-2 antibody [E17] ab32124 (purified) at 1/30 immunoprecipitating Bcl-2 in Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
All lanes: Immunoprecipitation - Anti-Bcl-2 antibody [E17] (Anti-Bcl-2 antibody [E17] ab32124)
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--western-blot-img5652.jpg "Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Bcl-2 antibody [E17] ab32124).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Bcl-2 antibody [E17] ab32124 observed at 26 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Bcl-2 antibody [E17] ab32124 was shown to react with Bcl-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BCL2 knockout HeLa cell line ab255364 (knockout cell lysate ab263752) was used. Wild-type HeLa and BCL2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Bcl-2 antibody [E17] ab32124 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Bcl-2 antibody [E17] (Anti-Bcl-2 antibody [E17] ab32124) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: BCL2 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81067.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Formaldehyde-fixed, paraffin-embedded human DLBCL U2932 cell line xenograft tissue stained for Bcl-2 using Anti-Bcl-2 antibody [E17] ab32124 at 1/200 dilution in immunohistochemical analysis, followed by Goat anti-Rabbit IgG Alexa Fluor® 555.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
![Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--western-blot-img137004.jpg "Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Bcl-2 antibody [E17] ab32124).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Bcl-2 antibody [E17] ab32124 observed at 26 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Bcl-2 antibody [E17] ab32124 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. Anti-Bcl-2 antibody [E17] ab32124 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. 3% milk used as blocking agent.
All lanes: Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: BCL2 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: THP-1 whole cell lysate at 20 µg
Predicted band size: 26 kDa
![Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--western-blot-img148241.jpg "Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
All lanes: MCF-7 (human breast carcinoma) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Predicted band size: 26 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136833.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B-cell lymphoma tissue labelling Bcl-2 with purified Anti-Bcl-2 antibody [E17] ab32124 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36181.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Bcl-2 with unpurified Anti-Bcl-2 antibody [E17] ab32124 at 1/200 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125895.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B cell lymphoma tissue labelling Bcl-2 with unpurified Anti-Bcl-2 antibody [E17] ab32124.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-2 antibody [E17] ab32124).
![OI-RD Scanning - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--oi-rd-scanning-img67905.jpg "OI-RD Scanning - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
OI-RD Scanning - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418320.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Bcl-2 antibody [E17] ab32124).
Tissue Microarrays for Anti-Bcl2 antibody [E17] using Anti-Bcl-2 antibody [E17] ab32124 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pretreated with heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The section was incubated with Anti-Bcl-2 antibody [E17] ab32124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--western-blot-img137004.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
![Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--western-blot-img148241.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136833.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36181.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125895.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
![OI-RD Scanning - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--oi-rd-scanning-img67905.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-e17-bsa-and-azide-free-ab185002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418320.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] - BSA and Azide free (ab185002)")
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