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Rat Recombinant Monoclonal Bcl-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-Bcl-2 antibody [NOR 235J] (AB241548), expandable thumbnail
  • Western blot - Anti-Bcl-2 antibody [NOR 235J] (AB241548), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] (AB241548), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Rat

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

0.98 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

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Target data

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Alternative names

Apoptosis regulator Bcl-2, BCL2

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Rat Recombinant Monoclonal Bcl-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

NOR 235J

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.

Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

Associated diseases and disorders

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

Product promise

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3 product images

  • Western blot - Anti-Bcl-2 antibody [NOR 235J] (ab241548), expandable thumbnail

    Western blot - Anti-Bcl-2 antibody [NOR 235J] (ab241548)

    Exposure time:

    Lane 1,2,5:3 seconds

    Lane 3,4:10 seconds

    Blocking buffer / Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Bcl-2 antibody [NOR 235J] (ab241548) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 2: U937 (human histiocytic lymphoma monocyte), Whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

    Lane 5: Human tonsil tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

  • Western blot - Anti-Bcl-2 antibody [NOR 235J] (ab241548), expandable thumbnail

    Western blot - Anti-Bcl-2 antibody [NOR 235J] (ab241548)

    Lanes 1 - 6: Merged signal (red and green). Green - ab241548 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

    ab241548 was shown to react with Bcl-2 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween) before incubation with ab241548 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat Anti-Rat IgG H&L (IRDye 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Bcl-2 antibody [NOR 235J] (ab241548) at 0.5 µg/mL

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: BCL2 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: NIH/3T3 whole cell lysate at 20 µg

    Lane 4: THP-1 whole cell lysate at 20 µg

    Lane 5: Human Spleen tissue lysate at 20 µg

    Lane 6: Mouse Spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rat IgG H&L (IRDye® 800CW ) (ab253031) at 1/20000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] (ab241548), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] (ab241548)

    IHC image of Bcl-2 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BOND™ system using the standard Protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab241548, 0.98 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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