Rat Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (AB252802), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-nor-235j-bsa-and-azide-free-ab252802--western-blot-img185639.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (AB252802)")
![Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (AB252802), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-nor-235j-bsa-and-azide-free-ab252802--western-blot-img164098.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (AB252802)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (AB252802), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-nor-235j-bsa-and-azide-free-ab252802--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134152.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (AB252802)")
Key facts
- Isotype
IgG2b
- Host species
Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.98 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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Target data
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Alternative names
Apoptosis regulator Bcl-2, BCL2
Recommended products
Rat Recombinant Monoclonal Bcl-2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG2b
- Host species
Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
NOR 235J
- Purification technique
Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab252802 is the carrier-free version of Anti-Bcl-2 antibody [NOR 235J] ab241548.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
- Biological function summary
The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.
- Pathways
Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.
- Associated diseases and disorders
The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-nor-235j-bsa-and-azide-free-ab252802--western-blot-img185639.jpg "Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)")
Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)
Exposure time:
Lane 1,2,5:3 seconds
Lane 3,4:10 seconds
Blocking buffer / Diluting buffer and concentration: 5% NFDM/TBST.
This image was produced using the same clone but in a different formulation, Anti-Bcl-2 antibody [NOR 235J] ab241548.
All lanes: Western blot - Anti-Bcl-2 antibody [NOR 235J] (Anti-Bcl-2 antibody [NOR 235J] ab241548) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Lane 2: U937 (human histiocytic lymphoma monocyte), Whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg
Lane 5: Human tonsil tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time:
Lane 1,2,5:3 seconds
Lane 3,4:10 seconds
Blocking buffer / Diluting buffer and concentration: 5% NFDM/TBST.
![Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-nor-235j-bsa-and-azide-free-ab252802--western-blot-img164098.jpg "Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)")
Western blot - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)
Lanes 1 - 6: Merged signal (red and green). Green - Anti-Bcl-2 antibody [NOR 235J] ab241548 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
Anti-Bcl-2 antibody [NOR 235J] ab241548 was shown to react with Bcl-2 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween) before incubation with Anti-Bcl-2 antibody [NOR 235J] ab241548 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat Anti-Rat IgG H&L (IRDye 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
This image was produced using the same clone but in a different formulation, Anti-Bcl-2 antibody [NOR 235J] ab241548.
All lanes: Western blot - Anti-Bcl-2 antibody [NOR 235J] (Anti-Bcl-2 antibody [NOR 235J] ab241548) at 0.5 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: BCL2 knockout HAP1 whole cell lysate at 20 µg
Lane 3: NIH/3T3 whole cell lysate at 20 µg
Lane 4: THP-1 whole cell lysate at 20 µg
Lane 5: Human Spleen tissue lysate at 20 µg
Lane 6: Mouse Spleen tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rat IgG H&L (IRDye® 800CW ) (ab253031) at 1/20000 dilution
Predicted band size: 26 kDa
Lanes 1 - 6: Merged signal (red and green). Green - Anti-Bcl-2 antibody [NOR 235J] ab241548 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
Anti-Bcl-2 antibody [NOR 235J] ab241548 was shown to react with Bcl-2 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween) before incubation with Anti-Bcl-2 antibody [NOR 235J] ab241548 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat Anti-Rat IgG H&L (IRDye 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802), expandable thumbnail](https://content.abcam.com/products/images/bcl-2-antibody-nor-235j-bsa-and-azide-free-ab252802--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134152.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [NOR 235J] - BSA and Azide free (ab252802)
This image was produced using the same clone but in a different formulation, Anti-Bcl-2 antibody [NOR 235J] ab241548.
IHC image of Bcl-2 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BOND™ system using the standard Protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Bcl-2 antibody [NOR 235J] ab241548, 0.98 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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