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Rabbit Recombinant Monoclonal Bcl-2 phospho S70 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 10 publications.

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Images

Western blot - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (AB218123), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (AB218123), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (AB218123), expandable thumbnail
  • Immunoprecipitation - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (AB218123), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (AB218123), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Bcl-2 phospho S70 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21162

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123), expandable thumbnail

    Western blot - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Blocking/Dilution buffer and concentration: 2% BSA/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 10097113).

    All lanes: Western blot - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123) at 1/5000 dilution

    Lane 1: Untreated Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: Jurkat treated with 1 μM paclitaxel for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa

    Exposure time: 32s

  • Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Bcl-2 (phospho S70) with ab218123 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/100 dilution (green). Confocal image showing positive staining in HeLa cells in M phase. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed,90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) treated with 1 μM paclitaxel for 24h (Right) / Untreated control (Left) cell line labeling Bcl-2 (phospho S70)with ab218123 at 1/500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Square gate shows Bcl-2 (phospho S70) positive signal. The expression profile is consistent with what has been described in the literature (PMID: 10097113)

  • Immunoprecipitation - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123), expandable thumbnail

    Immunoprecipitation - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Bcl-2 (phospho S70) was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cells from peripheral blood) treated with 1 μM paclitaxel for 24 hours whole cell lysate with ab218123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218123 at 0.5 μg/ml. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Jurkat treated with 1 μM paclitaxel for 24 hours whole cell lysate 10 μg (Input).

    Lane 2: ab218123 IP in Jurkat treated with 1 μM paclitaxel for 24 hours whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab218123 in Jurkat treated with 1 μM paclitaxel for 24 hours whole cell lysate.

    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 15 seconds.

    All lanes: Immunoprecipitation - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Predicted band size: 26 kDa

  • Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Intracellular flow cytometric analysis of 80% methanol-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Bcl-2 (phospho S70)with ab218123 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were pretreated with 20 μg/ml RNase A for 30 minutes to eliminate the non-specific binding between RNA and propidium iodide (PI).

    Bcl-2 (phospho S70) is highly expressed in mitotic cells (PMID: 10567572).

  • Dot Blot - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123), expandable thumbnail

    Dot Blot - Anti-Bcl-2 (phospho S70) antibody [EPR21162] (ab218123)

    Dot blot analysis of Bcl-2 (phospho S70) labeled with ab218123 at 1/1000 dilution.

    Lane 1: Bcl-2 (phospho S70) peptide.
    Lane 2: Bcl-2 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 2% BSA/TBST.

    Exposure time: 58 seconds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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