JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB73985

Anti-Bcl-2 (phospho S87) antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal Bcl-2 phospho S87 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2 phospho S87.

View Alternative Names

Apoptosis regulator Bcl-2, BCL2

2 Images
Western blot - Anti-Bcl-2 (phospho S87) antibody (AB73985)
  • WB

Unknown

Western blot - Anti-Bcl-2 (phospho S87) antibody (AB73985)

All lanes:

Western blot - Anti-Bcl-2 (phospho S87) antibody (ab73985) at 1/500 dilution

Lane 1:

Extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours) at 5 µg

Lane 2:

Extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours) at 5 µg with immunising phosphopeptide

Predicted band size: 26 kDa

Observed band size: 28 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 (phospho S87) antibody (AB73985)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 (phospho S87) antibody (AB73985)

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using ab73985 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BCL2 phospho S87. The exact immunogen used to generate this antibody is proprietary information.

P10415

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab73985 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).
See full target information BCL2 phospho S87
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Iranian journal of pharmaceutical research : IJPR 11:1285-90 PubMed24250563

2013

BCL-2 and Bax Expression in Skin Flaps Treated with Finasteride or Azelaic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Seyyed Abdulmajid Ayatollahi,Marjan Ajami,Hamed Reyhanfard,Yasin Asadi,Mansour Nassiri-Kashani,Mehdi Rashighi Firoozabadi,Sayed Hossein Davoodi,Esmaeil Habibi,Hamidreza Pazoki-Toroudi

The Journal of physiology 589:3349-69 PubMed21540338

2011

Protective effect of caspase inhibition on compression-induced muscle damage.

Applications

WB

Species

Rat

Bee T Teng,Eric W Tam,Iris F Benzie,Parco M Siu
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

primary-antibodies

bcl-2-antibody-epr17509-ab182858

5

(13 reviews)

Alternative Product
Primary Antibodies

AB233694

Anti-Bcl-2 (phospho S70) antibody [EPR21162] - BSA and Azide free

primary-antibodies

bcl-2-phospho-s70-antibody-epr21162-bsa-and-azide-free-ab233694

0

(0 reviews)

Alternative Product
Primary Antibodies

AB185002

Anti-Bcl-2 antibody [E17] - BSA and Azide free

primary-antibodies

bcl-2-antibody-e17-bsa-and-azide-free-ab185002

0

(0 reviews)

Alternative Product
Primary Antibodies

AB219608

Anti-Bcl-2 antibody [EPR17509] - BSA and Azide free

primary-antibodies

bcl-2-antibody-epr17509-bsa-and-azide-free-ab219608

0

(0 reviews)

Alternative Product
Primary Antibodies

AB32124

Anti-Bcl-2 antibody [E17]

primary-antibodies

bcl-2-antibody-e17-ab32124

3

(14 reviews)

Alternative Product
Primary Antibodies

AB218123

Anti-Bcl-2 (phospho S70) antibody [EPR21162]

primary-antibodies

bcl-2-phospho-s70-antibody-epr21162-ab218123

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com