Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)

Rabbit Recombinant Monoclonal Bcl G/BCL2L14 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Bcl G/BCL2L14 antibody [EPR17666] (AB184925), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Expected	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/40	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/130	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information BCL2L14

Function

Plays a role in apoptosis.

Alternative names

BCLG, BCL2L14, Apoptosis facilitator Bcl-2-like protein 14, Bcl2-L-14, Apoptosis regulator Bcl-G

Recommended products

Rabbit Recombinant Monoclonal Bcl G/BCL2L14 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17666
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Bcl G also known as BCL2L14 is a protein part of the Bcl-2 family closely related to apoptosis regulation. It has a molecular mass of approximately 45 kDa. Bcl G expresses in various tissues with significant levels reported in the testis and lower levels observed in several other tissues like the brain liver and prostate. The protein exists in isoforms known as Bcl-G(L) and Bcl-G(S) with the former being more widely expressed.

Biological function summary

The function of this protein involves the regulation of apoptosis. Bcl G functions as a pro-apoptotic protein playing a role in cell death processes. Studies show that it can exist as part of larger complexes interacting with other Bcl-2 family proteins. This interaction usually influences mitochondrial pathways of apoptosis where Bcl G aids in mitochondrial membrane permeability changes leading to cell death.

Pathways

Bcl G significantly participates in the apoptosis pathway and the mitochondrial outer membrane permeabilization process. This protein interacts with several key regulatory proteins including Bcl-2 and Bax. These interactions modulate the release of cytochrome c from mitochondria a critical step in the intrinsic apoptosis pathway. Bcl G also aligns with the pathway for p53-mediated apoptosis further linking it to the cellular stress response.

Associated diseases and disorders

Bcl G has associations with several forms of cancer. Research indicates a link between Bcl G expression and testicular cancer where it influences tumor progression and treatment response. In breast cancer Bcl G interacts with the well-known tumor suppressor p53 which suggests its role in tumor suppression and potential therapeutic target. Additionally mutations or altered expressions in this protein could disrupt normal apoptosis contributing to cancer development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Immunocytochemistry/ Immunofluorescence - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Bcl G/BCL2L14 with ab184925 at 1/250 dilution, followed by Goat anti-rabbit I Alexa Fluor® 488 (IgG) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184925 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925) at 1/2000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 2: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg
Lane 3: A549 (Human lung carcinoma) whole cell lysates at 20 µg
Lane 4: Human fetal liver lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 40 kDa
Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925) at 1/5000 dilution
All lanes: Human testis lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 40 kDa
Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925) at 1/2000 dilution
Lane 1: Mouse brain lysates at 10 µg
Lane 2: Mouse heart lysates at 10 µg
Lane 3: Mouse kidney lysates at 10 µg
Lane 4: Mouse spleen lysates at 10 µg
Lane 5: Rat brain lysates at 10 µg
Lane 6: Rat heart lysates at 10 µg
Lane 7: C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 8: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg
Lane 9: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg
Lane 10: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/500 dilution. Cytoplasm staining on lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
J Biol Chem. 2001 Jan 26;276(4):2780-5.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/500 dilution. Cytoplasmic staining on tumor cells of Human cervical squamous cell carcinoma is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Bcl G/BCL2L14 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab184925 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184925 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184925 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/500 dilution. Cytoplasmic staining on mouse kidney tubules is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Bcl G/BCL2L14 with ab184925 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/500 dilution. Cytoplasmic staining on glandular epithelium of rat colon is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Bcl G/BCL2L14 antibody [EPR17666] (ab184925)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat (Human T cell leukemia cells from peripheral blood) cells labeling Bcl G/BCL2L14 with ab184925 at 1/130 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.