Mouse Recombinant Monoclonal Bcl-XL antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--western-blot-img37096.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16114.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257)")
![Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--western-blot-img20858.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17157.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (AB252257)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | ICC | IP | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). Isoform Bcl-X(S) promotes apoptosis.
Alternative names
BCL2L, BCLX, BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X
Recommended products
Mouse Recombinant Monoclonal Bcl-XL antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 2H12
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab252257 is the carrier-free version of Anti-Bcl-XL antibody [2H12] ab77571.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.
- Biological function summary
This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.
- Pathways
The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.
- Associated diseases and disorders
Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--western-blot-img37096.jpg "Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)")
Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-XL antibody [2H12] ab77571).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Bcl-XL antibody [2H12] (Anti-Bcl-XL antibody [2H12] ab77571) at 1/1000 dilution
Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 10 µg
Lane 2: HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16114.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Bcl-XL with Anti-Bcl-XL antibody [2H12] ab77571 at 1/100 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on human kidney. The section was incubated with Anti-Bcl-XL antibody [2H12] ab77571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-XL antibody [2H12] ab77571).
![Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--western-blot-img20858.jpg "Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)")
Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-XL antibody [2H12] ab77571).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 37 seconds.
All lanes: Western blot - Anti-Bcl-XL antibody [2H12] (Anti-Bcl-XL antibody [2H12] ab77571) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-2h12-bsa-and-azide-free-ab252257--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17157.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling Bcl-XL with Anti-Bcl-XL antibody [2H12] ab77571 at 1/100 dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on human colon carcinoma. The section was incubated with Anti-Bcl-XL antibody [2H12] ab77571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl-XL antibody [2H12] ab77571).
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