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AB98143

Anti-Bcl-XL antibody

4

(2 Reviews)

|

(8 Publications)

Rabbit Polyclonal Bcl-XL antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BCL2L1 aa 1-200.

View Alternative Names

BCL2L, BCLX, BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (AB98143)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (AB98143)

Immunohistochemical analysis of Bcl x in paraffin embedded SW480 xenograft, using ab98143 at a 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Bcl-XL antibody (AB98143)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcl-XL antibody (AB98143)

Immunofluorescence analysis of SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min labelling Bcl-X protein at cytoplasm using ab98143 at a 1/200 dilution (Green). alpha Tubulin, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining.

Immunoprecipitation - Anti-Bcl-XL antibody (AB98143)
  • IP

Supplier Data

Immunoprecipitation - Anti-Bcl-XL antibody (AB98143)

Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 µg of Bcl-X antibody.
Western blot analysis was performed using Bcl-X antibody at a 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-Bcl-XL antibody (ab98143) at 5 µg

Lane 1:

HCT-116 whole cell extracts with control IgG

Lane 2:

HCT-116 whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG Antibody

Observed band size: 30 kDa

false

Western blot - Anti-Bcl-XL antibody (AB98143)
  • WB

Supplier Data

Western blot - Anti-Bcl-XL antibody (AB98143)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution

Lane 1:

HepG2 extracts at 30 µg

Lane 2:

HepG2 mitochondria extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 30 kDa

false

Western blot - Anti-Bcl-XL antibody (AB98143)
  • WB

Unknown

Western blot - Anti-Bcl-XL antibody (AB98143)

12% SDS PAGE

All lanes:

Western blot - Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BCL2L1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q07817

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.
Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).. Isoform Bcl-X(S) promotes apoptosis.
See full target information BCL2L1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 22: PubMed38921561

2024

Marine Cytotoxin Santacruzamate A Derivatives as Potent HDAC1-3 Inhibitors and Their Synergistic Anti-Leukemia Effects with Venetoclax.

Applications

Unspecified application

Species

Unspecified reactive species

Wanting Hao,Leyan Wang,Tongqiang Xu,Geng Jia,Yuqi Jiang,Chong Qin,Xiaoyang Li

Cancer science 114:3318-3329 PubMed37302808

2023

WDR54 exerts oncogenic roles in T-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Li,Danlan Zhang,Qiuxia Fu,Shang Wang,Xin Zhang,Zhixian Lin,Zhongyuan Wang,Jiaxing Song,Zijie Su,VivianWeiwen Xue,Shanshan Liu,Yun Chen,Liang Zhou,Na Zhao,Desheng Lu

Cancer medicine 12:15650-15663 PubMed37293953

2023

BCL11B expression in hepatocellular carcinoma relates to chemosensitivity and clinical prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Abe,Kenya Kamimura,Shujiro Okuda,Yu Watanabe,Jun Inoue,Yutaka Aoyagi,Toshifumi Wakai,Ryo Kominami,Shuji Terai

International journal of molecular medicine 47: PubMed33448314

2021

Harnessing adipose‑derived stem cells to release specialized secretome for the treatment of hepatitis B.

Applications

Unspecified application

Species

Unspecified reactive species

Hee Ju Kim,Ok-Hee Kim,Ha-Eun Hong,Sang Chul Lee,Say-June Kim

BioMed research international 2020:4926821 PubMed33415148

2020

Effect of Calomelanone, a Dihydrochalcone Analogue, on Human Cancer Apoptosis/Regulated Cell Death in an Model.

Applications

Unspecified application

Species

Unspecified reactive species

Wasitta Rachakhom,Ratana Banjerdpongchai

Cancer biology & therapy 21:452-462 PubMed32089062

2020

Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Li,Xingtao Han,Pengtao Wei,Jinhui Yang,Jiantao Sun

Molecular cancer therapeutics 17:2575-2585 PubMed30266802

2018

Dual Targeting of Aurora Kinases with AMG 900 Exhibits Potent Preclinical Activity Against Acute Myeloid Leukemia with Distinct Post-Mitotic Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Payton,Hung-Kam Cheung,Maria Stefania S Ninniri,Christian Marinaccio,William C Wayne,Kelly Hanestad,John D Crispino,Gloria Juan,Angela Coxon

International journal of clinical and experimental 4:616-27 PubMed21904637

2011

Brain microvasculature and hypoxia-related proteins in Alzheimer's disease.

Applications

WB

Species

Rat

Paula Grammas,Debjani Tripathy,Alma Sanchez,Xiangling Yin,Jinhua Luo
View all publications
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Product promise

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