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Rabbit Recombinant Monoclonal Bcl-XL antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

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Images

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (AB178844), expandable thumbnail
  • Western blot - Anti-Bcl-XL antibody [EPR16642] (AB178844), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Not recommended
Tested
Rat
Expected
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/70

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/20000

Notes

-

Species

Rat

Dilution info

1/20000

Notes

-

Species

Human

Dilution info

1/20000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).Isoform Bcl-X(S) promotes apoptosis.

Alternative names

Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L, BCLX, BCL2L1

Recommended products

Rabbit Recombinant Monoclonal Bcl-XL antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

Alternative names

Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L, BCLX, BCL2L1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16642

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.

Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Associated diseases and disorders

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.

    Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration:

    5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Predicted band size: 26 kDa

  • Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844) at 1/20000 dilution

    Lane 1: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1:2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Cytoplasm staining on epithelial cells of mouse colon tissue is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Cytoplasm staining on epithelial cells of testis is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Blocking buffer and concentration: 5% NFDM/TBST

    All lanes: Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate

    Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lane 4: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Secondary

    All lanes: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 30 kDa

  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    Predicted band size: 26 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Blocking buffer and concentration: 5% NFDM/TBST

    All lanes: Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1: Mouse brain

    Lane 2: Mouse heart

    Lane 3: Mouse kidney

    Lane 4: Mouse spleen

    Lane 5: Rat brain

    Lane 6: Rat heart

    Lane 7: Rat kidney

    Lane 8: Rat spleen

    Lane 9: C6 whole cell lysates

    Lane 10: Raw264.7 whole cell lysates

    Lane 11: PC-12 whole cell lysates

    Lane 12: NIH/3T3 whole cell lysates

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 26 kDa

    Observed band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (ab178844)

    This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

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