AB223547

Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Bcl-XL antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

BCL2L, BCLX, BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1 : 2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.

Negative control : Using PBS instead of primary ab, secondary ab as above.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
Negative control : Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Ramos whole cell extract. Right lane : PBS instead of Ramos whole cell extract.

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (<a href='/en-us/products/primary-antibodies/bcl-xl-antibody-epr16642-ab178844'>ab178844</a>)

Predicted band size: 26 kDa

false

Lab

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Ramos whole cell extract. Right lane : PBS instead of Ramos whole cell extract.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (<a href='/en-us/products/primary-antibodies/bcl-xl-antibody-epr16642-ab178844'>ab178844</a>)

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

Cytoplasm staining on epithelial cells of mouse colon tissue is observed.

Negative control : Using PBS instead of primary ab, secondary ab as above.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Cytoplasm staining on epithelial cells of testis is observed.

Negative control : Using PBS instead of primary ab, secondary ab as above.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
Negative control : Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

All lanes:

Anti-Bcl-XL/S [EPR16642] antibody (<a href='/en-us/products/primary-antibodies/bcl-xl-antibody-epr16642-ab178844'>ab178844</a>) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lane 4:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa

true

Supplier Data

Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Bcl-XL antibody [EPR16642] (<a href='/en-us/products/primary-antibodies/bcl-xl-antibody-epr16642-ab178844'>ab178844</a>) at 1/20000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

true

Lab

Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

This data was developed using ab178844, the same antibody clone in a different buffer formulation.

This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Lane 1:

Mouse brain

Lane 2:

Mouse heart

Lane 3:

Mouse kidney

Lane 4:

Mouse spleen

Lane 5:

Rat brain

Lane 6:

Rat heart

Lane 7:

Rat kidney

Lane 8:

Rat spleen

Lane 9:

C6 whole cell lysates

Lane 10:

Raw264.7 whole cell lysates

Lane 11:

PC-12 whole cell lysates

Lane 12:

NIH/3T3 whole cell lysates

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

Unconjugated

Anti-Bcl-XL antibody [EPR16642]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16642

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab223547 is the carrier-free version of ab178844.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.

Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).. Isoform Bcl-X(S) promotes apoptosis.

See full target information BCL2L1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1213-1227 PubMed40259083

2025

Multi-omic and spatial analysis of mouse kidneys highlights sex-specific differences in gene regulation across the lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Chen,Ruiyang Liu,Chia-Kuei Mo,Michael C Wendl,Andrew Houston,Preet Lal,Yanyan Zhao,Wagma Caravan,Andrew T Shinkle,Atieh Abedin-Do,Nataly Naser Al Deen,Kazuhito Sato,Xiang Li,André Luiz N Targino da Costa,Yize Li,Alla Karpova,John M Herndon,Maxim N Artyomov,Joshua B Rubin,Sanjay Jain,Xue Li,Sheila A Stewart,Li Ding,Feng Chen

Journal of biochemical and molecular toxicology 36:e23196 PubMed35979984

2022

PEX1 is a mediator of α1-adrenergic signaling attenuating doxorubicin-induced cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Li,Huilin Xie,Huang Hu,Jihong Huang,Sun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise