Rabbit Recombinant Monoclonal Bcl-XL antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunoprecipitation-img482.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)")
![Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--western-blot-img99774.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114626.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109960.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119041.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (AB223547)")
Publications
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- Journal of biochemical and molecular toxicology 36:e231962022PEX1 is a mediator of α1-adrenergic signaling attenuating doxorubicin-induced cardiotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjuan Li,Huilin Xie,Huang Hu,Jihong Huang,Sun ChenPubMed 35979984
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested | Not recommended | Tested |
| Rat | Expected | Not recommended | Tested | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).Isoform Bcl-X(S) promotes apoptosis.
Alternative names
Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L, BCLX, BCL2L1
Recommended products
Rabbit Recombinant Monoclonal Bcl-XL antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Alternative names
Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L, BCLX, BCL2L1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR16642
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab223547 is the carrier-free version of Anti-Bcl-XL antibody [EPR16642] ab178844.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.
- Biological function summary
This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.
- Pathways
The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.
- Associated diseases and disorders
Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunoprecipitation-img482.jpg "Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with Anti-Bcl-XL antibody [EPR16642] ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using Anti-Bcl-XL antibody [EPR16642] ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration:
5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (Anti-Bcl-XL antibody [EPR16642] ab178844)
Predicted band size: 26 kDa
![Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--western-blot-img99774.jpg "Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Bcl-XL antibody [EPR16642] (Anti-Bcl-XL antibody [EPR16642] ab178844) at 1/20000 dilution
Lane 1: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114626.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with Anti-Bcl-XL antibody [EPR16642] ab178844 at 1:2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109960.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with Anti-Bcl-XL antibody [EPR16642] ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119041.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with Anti-Bcl-XL antibody [EPR16642] ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of testis is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--western-blot-img38365.jpg "Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
This western blot image is a comparison between Anti-Bcl-XL antibody [EPR16642] ab178844 and a competitor's leading rabbit polyclonal antibody.
All lanes: Anti-Bcl-XL/S [EPR16642] antibody (Anti-Bcl-XL antibody [EPR16642] ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution
Lane 1: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 30 kDa
![Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunoprecipitation-img144966.jpg "Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.
This immunoprecipitation image is a comparison between Anti-Bcl-XL antibody [EPR16642] ab178844 and a competitor's leading rabbit polyclonal antibody.
Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with Anti-Bcl-XL antibody [EPR16642] ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using Anti-Bcl-XL antibody [EPR16642] ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (Anti-Bcl-XL antibody [EPR16642] ab178844)
Predicted band size: 26 kDa
Observed band size: 30 kDa
![Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--western-blot-img120384.jpg "Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.
This western blot image is a comparison between Anti-Bcl-XL antibody [EPR16642] ab178844 and a competitor's leading rabbit polyclonal antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Bcl-XL/S [EPR16642] antibody (Anti-Bcl-XL antibody [EPR16642] ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution
Lane 1: Mouse brain
Lane 2: Mouse heart
Lane 3: Mouse kidney
Lane 4: Mouse spleen
Lane 5: Rat brain
Lane 6: Rat heart
Lane 7: Rat kidney
Lane 8: Rat spleen
Lane 9: C6 whole cell lysates
Lane 10: Raw264.7 whole cell lysates
Lane 11: PC-12 whole cell lysates
Lane 12: NIH/3T3 whole cell lysates
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 26 kDa
Observed band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66146.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between Anti-Bcl-XL antibody [EPR16642] ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with Anti-Bcl-XL antibody [EPR16642] ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
This data was developed using Anti-Bcl-XL antibody [EPR16642] ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between Anti-Bcl-XL antibody [EPR16642] ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with Anti-Bcl-XL antibody [EPR16642] ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--western-blot-img38365.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
![Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunoprecipitation-img144966.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
![Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--western-blot-img120384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66146.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547), expandable thumbnail](https://content.abcam.com/products/images/bcl-xl-antibody-epr16642-bsa-and-azide-free-ab223547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)")
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