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Rabbit Polyclonal BCL10 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human BCL10 aa 1 to C-terminus.

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Images

Western blot - Anti-Bcl10 antibody (AB155657), expandable thumbnail
  • Western blot - Anti-Bcl10 antibody (AB155657), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (AB155657), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human BCL10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95999

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted
Cow
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation (PubMed:10187770, PubMed:10364242, PubMed:10400625, PubMed:25365219, PubMed:24074955). Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:24074955). Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex (PubMed:24074955). This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:18287044, PubMed:27777308, PubMed:24074955). Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity (PubMed:26488816). Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR) (PubMed:18264101, PubMed:18287044, PubMed:27777308, PubMed:24074955). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK (PubMed:10187815).

Alternative names

Recommended products

Rabbit Polyclonal BCL10 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human BCL10 aa 1 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human BCL10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95999
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Bcl10 also known as B-cell lymphoma 10 is a protein involved in several cellular processes. It has a molecular mass of approximately 26 kDa and shows expression in various lymphoid tissues. Bcl10 plays a significant role in signaling pathways by transmitting signals from the cell surface to the nucleus impacting cell growth and immune responses. Researchers often study Bcl10 using immunohistochemistry (IHC) techniques referred to as Bcl10 IHC to analyze its expression and distribution within tissues.

Biological function summary

Bcl10 activates downstream signaling pathways that are critical for immune cell functioning and survival. It forms part of the CARD-BCL10-MALT1 (CBM) complex which is important for the activation of the NF-kB pathway. This complex formation is essential for lymphocyte activation and proliferation. Bcl10 interactions within this complex influence cellular responses to various stimuli helping organisms to mount appropriate immune responses.

Pathways

Bcl10 serves as an integral component in the NF-kB and TCR signaling pathways. It functions alongside proteins such as MALT1 and CARD9 in these pathways. The proper functioning of Bcl10 in these pathways ensures accurate transcriptional regulation necessary for immune responses. Misregulation in these pathways can lead to immune disorders and aberrant cell behavior linking Bcl10 to further biological implications.

Associated diseases and disorders

Bcl10 shows associations with mucosa-associated lymphoid tissue (MALT) lymphoma and other inflammatory diseases. Aberrant Bcl10 expression or mutations can lead to unchecked activation of NF-kB contributing to the progression of these conditions. In MALT lymphoma Bcl10 often cooperates with MALT1 both contributing to pathological cell survival and proliferation highlighting the importance of Bcl10 in understanding these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-Bcl10 antibody (ab155657), expandable thumbnail

    Western blot - Anti-Bcl10 antibody (ab155657)

    12% SDS PAGE

    All lanes: Western blot - Anti-Bcl10 antibody (ab155657) at 1/1000 dilution

    Lane 1: HepG2 whole cell lysate at 30 µg

    Lane 2: MOLT4 whole cell lysate at 30 µg

    Lane 3: Raji whole cell lysate at 30 µg

    Predicted band size: 26 kDa

  • Western blot - Anti-Bcl10 antibody (ab155657), expandable thumbnail

    Western blot - Anti-Bcl10 antibody (ab155657)

    12% SDS PAGE

    All lanes: Western blot - Anti-Bcl10 antibody (ab155657) at 1/1000 dilution

    All lanes: mouse NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (ab155657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (ab155657)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SAS xenograft tissue labeling Bcl10 using ab155657 at a 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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