AB227206

Anti-Bcl10 antibody

Name: Anti-Bcl10 antibody (ab227206) | Abcam
Brand: Abcam Limited
SKU: AB227206
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal BCL10 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BCL10.

View Alternative Names

CIPER, CLAP, BCL10, B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN, Cellular-E10, Mammalian CARD-containing adapter molecule E10, Bcl-10, hCLAP, cCARMEN, c-E10, mE10

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody (AB227206)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody (AB227206)

2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bcl10 (green) using ab227206 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (AB227206)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (AB227206)

Paraffin-embedded human gastric cancer tissue stained for Bcl10 using ab227206 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-Bcl10 antibody (AB227206)

Bcl10 was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab227206. Western blot was performed from the immunoprecipitate using ab227206 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent

Lane 1 : Raji whole cell extract 40 μg (Input).

Lane 2 : Control IP in Raji whole cell extract with 4 μg of preimmune Rabbit IgG.

Lane 3 : ab227206 IP in Raji whole cell extract.

All lanes:

Immunoprecipitation - Anti-Bcl10 antibody (ab227206)

Predicted band size: 26 kDa

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Lab

Western blot - Anti-Bcl10 antibody (AB227206)

All lanes:

Western blot - Anti-Bcl10 antibody (ab227206) at 1/2500 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

Bcl10 Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-Bcl10 antibody (AB227206)

12% SDS PAGE gel.

All lanes:

Western blot - Anti-Bcl10 antibody (ab227206) at 1/1000 dilution

All lanes:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-Bcl10 antibody (AB227206)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Bcl10 antibody (ab227206) at 1/2500 dilution

Lane 1:

Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Lane 2:

Bcl10 knockout HeLa cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-Bcl10 antibody (AB227206)

12 % SDS-PAGE gel.

All lanes:

Western blot - Anti-Bcl10 antibody (ab227206) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BCL10. The exact immunogen used to generate this antibody is proprietary information.

O95999

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl10 also known as B-cell lymphoma 10 is a protein involved in several cellular processes. It has a molecular mass of approximately 26 kDa and shows expression in various lymphoid tissues. Bcl10 plays a significant role in signaling pathways by transmitting signals from the cell surface to the nucleus impacting cell growth and immune responses. Researchers often study Bcl10 using immunohistochemistry (IHC) techniques referred to as Bcl10 IHC to analyze its expression and distribution within tissues.

Biological function summary

Bcl10 activates downstream signaling pathways that are critical for immune cell functioning and survival. It forms part of the CARD-BCL10-MALT1 (CBM) complex which is important for the activation of the NF-kB pathway. This complex formation is essential for lymphocyte activation and proliferation. Bcl10 interactions within this complex influence cellular responses to various stimuli helping organisms to mount appropriate immune responses.

Pathways

Bcl10 serves as an integral component in the NF-kB and TCR signaling pathways. It functions alongside proteins such as MALT1 and CARD9 in these pathways. The proper functioning of Bcl10 in these pathways ensures accurate transcriptional regulation necessary for immune responses. Misregulation in these pathways can lead to immune disorders and aberrant cell behavior linking Bcl10 to further biological implications.

Bcl10 shows associations with mucosa-associated lymphoid tissue (MALT) lymphoma and other inflammatory diseases. Aberrant Bcl10 expression or mutations can lead to unchecked activation of NF-kB contributing to the progression of these conditions. In MALT lymphoma Bcl10 often cooperates with MALT1 both contributing to pathological cell survival and proliferation highlighting the importance of Bcl10 in understanding these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation (PubMed : 10187770, PubMed : 10364242, PubMed : 10400625, PubMed : 24074955, PubMed : 25365219). Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed : 24074955). Recruited by activated CARD domain-containing proteins : homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex (PubMed : 24074955). This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed : 18287044, PubMed : 24074955, PubMed : 27777308). Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity (PubMed : 26488816). Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR) (PubMed : 18264101, PubMed : 18287044, PubMed : 24074955, PubMed : 27777308). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK (PubMed : 10187815).

See full target information BCL10

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 11:e42095 PubMed39916855

2025

Dihydroartemisinin inhibits EphA2/PI3K/Akt pathway-mediated malignant behaviors and vasculogenic mimicry in glioma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huangde Fu,Shengtian Wu,Hechun Shen,Kai Luo,Zhongxiang Huang,Nankun Lu,Yaolin Li,Qian Lan,Yishun Xian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Bcl10 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody (AB227206)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (AB227206)

Immunoprecipitation - Anti-Bcl10 antibody (AB227206)

Western blot - Anti-Bcl10 antibody (AB227206)

Western blot - Anti-Bcl10 antibody (AB227206)

Western blot - Anti-Bcl10 antibody (AB227206)

Western blot - Anti-Bcl10 antibody (AB227206)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise