AB251397

Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Bcl2-L-13 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

MIL1, CD003, BCL2L13, Bcl-2-like protein 13, Bcl2-L-13, Bcl-rambo, Protein Mil1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

This data was developed using ab203516, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on human pancreas tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

This data was developed using ab203516, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic and nuclear staining on human tonsil tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

This data was developed using ab203516, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Bcl2-L-13 antibody [EP10625] (<a href='/en-us/products/primary-antibodies/bcl2-l-13-antibody-ep10625-ab203516'>ab203516</a>) at 1/5000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg

Lane 2:

THP-1 (Human monocytic leukemia cell line) cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 85 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

This data was developed using ab203516, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Bcl2-L-13 antibody [EP10625] (<a href='/en-us/products/primary-antibodies/bcl2-l-13-antibody-ep10625-ab203516'>ab203516</a>) at 1/5000 dilution

All lanes:

Raji (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 85 kDa

false

Exposure time: 3min

Unconjugated

Anti-Bcl2-L-13 antibody [EP10625]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP10625

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251397 is the carrier-free version of ab203516.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl2-L-13 also known as Bcl-rambo or BCL2-LIKE 13 is a member of the Bcl-2 protein family with a molecular mass of approximately 55 kDa. This protein is involved in the regulation of apoptosis a process of programmed cell death. Bcl2-L-13 is a membrane protein mainly expressed in the mitochondria but can also be found in other cellular compartments like the endoplasmic reticulum. Its expression levels and activity can vary among different tissues and under different conditions.

Biological function summary

Bcl2-L-13 regulates mitochondrial dynamics and influences the mitochondrial apoptotic machinery. It can act as a pro-apoptotic or anti-apoptotic factor depending on its interactions with other Bcl-2 family proteins. This protein does not work alone but often forms complexes with other Bcl-2 family members to modulate apoptotic pathways. By affecting mitochondrial membrane permeabilization it controls the release of cytochrome c and other pro-apoptotic factors that trigger downstream caspase cascades.

Pathways

Bcl2-L-13 is involved in intrinsic apoptosis signaling and mitochondrial maintenance pathways. It interacts with proteins like Bax and Bak facilitating either protection against apoptosis or promotion of cell death. Additionally Bcl2-L-13 influences crosstalk between the mitochondria and other cellular organelles affecting cell survival and death mechanisms under stress conditions. These interactions play a significant role in maintaining cellular homeostasis.

Bcl2-L-13 has been linked to various cancers and neurodegenerative diseases. Changes in its expression or function can lead to aberrant apoptosis contributing to tumor growth and resistance to chemotherapy. In neurodegenerative disorders such as Alzheimer's disease Bcl2-L-13 influences neuronal apoptosis pathways often in association with other proteins like APP or presenilin. Understanding the role of Bcl2-L-13 in these conditions helps develop targeted therapies aimed at modulating its activity to restore normal apoptotic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May promote the activation of caspase-3 and apoptosis.

See full target information BCL2L13

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (AB251397)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise