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Mouse Monoclonal BCL2L10 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BCL2L10 aa 1-200.

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Images

Western blot - Anti-BCL2L10 antibody [8A2F9] (AB233641), expandable thumbnail
  • Flow Cytometry - Anti-BCL2L10 antibody [8A2F9] (AB233641), expandable thumbnail
  • Western blot - Anti-BCL2L10 antibody [8A2F9] (AB233641), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BCL2L10 antibody [8A2F9] (AB233641), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (AB233641), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human BCL2L10 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HD36

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/400.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Target data

Function

Promotes cell survival by suppressing apoptosis induced by BAX but not BAK (PubMed:11278245, PubMed:11689480). Increases binding of AHCYL1/IRBIT to ITPR1 (PubMed:27995898). Reduces ITPR1-mediated calcium release from the endoplasmic reticulum cooperatively with AHCYL1/IRBIT under normal cellular conditions (PubMed:27995898). Under apoptotic stress conditions, dissociates from ITPR1 and is displaced from mitochondria-associated endoplasmic reticulum membranes, leading to increased Ca(2+) transfer to mitochondria which promotes apoptosis (PubMed:27995898). Required for the correct formation of the microtubule organizing center during oocyte cell division, potentially via regulation of protein abundance and localization of other microtubule organizing center components such as AURKA and TPX2 (By similarity).

Alternative names

BCL-B, BCLB, BOO, DIVA, BCL2L10, Bcl-2-like protein 10, Bcl2-L-10, Anti-apoptotic protein Boo, Anti-apoptotic protein NrH, Apoptosis regulator Bcl-B

Recommended products

Mouse Monoclonal BCL2L10 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BCL2L10 aa 1-200.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human BCL2L10 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HD36
Clone number
8A2F9
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641), expandable thumbnail

    Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Expected MW is 43.1 kDa.

    All lanes: Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641) at 1/500 dilution

    All lanes: Human BCL2L10 (aa 31-186) recombinant protein

    Predicted band size: 22 kDa

  • Flow Cytometry - Anti-BCL2L10 antibody [8A2F9] (ab233641), expandable thumbnail

    Flow Cytometry - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BCL2L10 with ab233641 at 1/200 dilution (green) compared with a negative control (red).

  • Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641), expandable thumbnail

    Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    All lanes: Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641) at 1/500 dilution

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lane 2: BCL2L10 (aa 31-186)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BCL2L10 antibody [8A2F9] (ab233641), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BCL2L10 (green) using ab233641 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Paraffin-embedded human lung cancer tissue stained for BCL2L10 using ab233641 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Paraffin-embedded human cervical cancer tissue stained for BCL2L10 using ab233641 at 1/200 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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