AB27780

Anti-Bcl3 antibody

(2 Reviews)

(4 Publications)

Rabbit Polyclonal BCL3 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human BCL3.

View Alternative Names

BCL4, D19S37, BCL3, B-cell lymphoma 3 protein, BCL-3, Proto-oncogene BCL3

1 Images

Unknown

Western blot - Anti-Bcl3 antibody (AB27780)

All lanes:

Western blot - Anti-Bcl3 antibody (ab27780) at 2 µg/mL

All lanes:

Jurkat

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human BCL3. The exact immunogen used to generate this antibody is proprietary information.

P20749

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3407 Bcl-3 Antibody. 3407-100 is the same size as the 100 μg size of ab27780.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Affinity purified

Storage buffer

pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl3 protein also known as Bcl-3 is a member of the IκB family of proteins. It serves a mechanical role as a transcription coactivator that interacts with NF-κB p50 and p52 homodimers. The Bcl3 protein has a molecular weight of approximately 45 kDa. This protein expresses primarily in lymphoid tissues including spleen and lymph nodes usually found within the nucleus where it performs its function in regulating gene expression.

Biological function summary

Bcl3 interacts with specific components of the NF-κB complex and modulates signaling pathways involved in immune responses inflammation and cell proliferation. Bcl3 helps in the stabilization and activation of p50/p52 dimers which facilitate the transcription of target genes. Its role necessitates its function within larger complexes that are essential for transcriptional regulation. Bcl3's interaction with other transcription factors allows it to fine-tune the expression of a range of genes impacting several cellular processes.

Pathways

Bcl3 plays a significant role in the regulation of the NF-κB signaling pathway. It acts by modulating the activity of NF-κB dimers important for immune response and inflammation. Furthermore it affects the PI3K/Akt pathway by influencing the expression of genes that are relevant to cell survival and apoptosis. Proteins such as p50 and p52 in the NF-κB pathway closely relate to Bcl3 function highlighting its role in cellular signaling networks.

Bcl3 is linked to certain types of cancers and autoimmune diseases. Its abnormal expression associates with disorders like chronic lymphocytic leukemia where it may contribute to disease progression by affecting cell proliferation and survival. Bcl3's interaction with NF-κB components can also influence rheumatoid arthritis where it might play a part in exacerbating inflammation. Proteins such as p50 and other NF-κB pathway components connect Bcl3 to these diseases reflecting its importance in disease mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).

See full target information BCL3

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastric cancer 20:95-105 PubMed32269848

2020

The Influence of Bcl-3 Expression on Cell Migration and Chemosensitivity of Gastric Cancer Cells via Regulating Hypoxia-Induced Protective Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Hu,Zhigang Bai,Xuemei Ma,Nan Bai,Zhongtao Zhang

Journal of cellular and molecular medicine 24:202-213 PubMed31668012

2019

Knockdown of long non-coding RNA LINC00176 suppresses ovarian cancer progression by BCL3-mediated down-regulation of ceruloplasmin.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Dai,Jixiang Niu,Yanli Feng

Molecular medicine reports 17:4832-4838 PubMed29328450

2018

MYBL2 protects against H9c2 injury induced by hypoxia via AKT and NF‑κB pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mingfeng Shao,Zexiang Ren,Rongjun Zhang

Brazilian journal of medical and biological resear 50:e5861 PubMed28355355

2017

CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

T Y Long,R Jing,F Kuang,L Huang,Z X Qian,T L Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl3 antibody

Western blot - Anti-Bcl3 antibody (AB27780)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise