AB259832

Anti-Bcl3 antibody [EPR23835-115]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal BCL3 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

BCL4, D19S37, BCL3, B-cell lymphoma 3 protein, BCL-3, Proto-oncogene BCL3

2 Images

Lab

Western blot - Anti-Bcl3 antibody [EPR23835-115] (AB259832)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Fresh lysate was used in lane 1.

Exposure time : Lane 1 : 5.5 seconds; Lane 2-3 : 3 minutes.

All lanes:

Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-Bcl3 antibody [EPR23835-115] (AB259832)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : THP-1 (PMID : 25089799).

Exposure time : 81 seconds.

All lanes:

Western blot - Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 50 kDa

false

Carrier free

Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23835-115

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl3 protein also known as Bcl-3 is a member of the IκB family of proteins. It serves a mechanical role as a transcription coactivator that interacts with NF-κB p50 and p52 homodimers. The Bcl3 protein has a molecular weight of approximately 45 kDa. This protein expresses primarily in lymphoid tissues including spleen and lymph nodes usually found within the nucleus where it performs its function in regulating gene expression.

Biological function summary

Bcl3 interacts with specific components of the NF-κB complex and modulates signaling pathways involved in immune responses inflammation and cell proliferation. Bcl3 helps in the stabilization and activation of p50/p52 dimers which facilitate the transcription of target genes. Its role necessitates its function within larger complexes that are essential for transcriptional regulation. Bcl3's interaction with other transcription factors allows it to fine-tune the expression of a range of genes impacting several cellular processes.

Pathways

Bcl3 plays a significant role in the regulation of the NF-κB signaling pathway. It acts by modulating the activity of NF-κB dimers important for immune response and inflammation. Furthermore it affects the PI3K/Akt pathway by influencing the expression of genes that are relevant to cell survival and apoptosis. Proteins such as p50 and p52 in the NF-κB pathway closely relate to Bcl3 function highlighting its role in cellular signaling networks.

Bcl3 is linked to certain types of cancers and autoimmune diseases. Its abnormal expression associates with disorders like chronic lymphocytic leukemia where it may contribute to disease progression by affecting cell proliferation and survival. Bcl3's interaction with NF-κB components can also influence rheumatoid arthritis where it might play a part in exacerbating inflammation. Proteins such as p50 and other NF-κB pathway components connect Bcl3 to these diseases reflecting its importance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).

See full target information BCL3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Genetics and molecular biology 47:e20230231 PubMed38577985

2024

LTBP2 regulates cisplatin resistance in GC cells via activation of the NF-κB2/BCL3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Wenjia Liang,Xiangwen Wang,Zhao Chen,Lei Jiang

Journal of orthopaedic translation 35:72-80 PubMed36186660

2022

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fuxiao Wang,Jiawei Guo,Yili Wang,Yan Hu,Hao Zhang,Jiao Chen,Yingying Jing,Liehu Cao,Xiao Chen,Jiacan Su

Clinical and translational medicine 12:e955 PubMed35804493

2022

B-cell lymphoma-3 controls mesenchymal stem cell commitment and senescence during skeletal aging.

Applications

Unspecified application

Species

Unspecified reactive species

Fuxiao Wang,Jiawei Guo,Sicheng Wang,Yili Wang,Jiao Chen,Yan Hu,Hao Zhang,Ke Xu,Yan Wei,Liehu Cao,Xiao Chen,Yingying Jing,Jiacan Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl3 antibody [EPR23835-115]

Western blot - Anti-Bcl3 antibody [EPR23835-115] (AB259832)

Western blot - Anti-Bcl3 antibody [EPR23835-115] (AB259832)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise