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AB15046

Anti-Bcl3 antibody [Ham150-3.5]

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(1 Publication)

Hamster Monoclonal BCL3 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Mouse Bcl3.

View Alternative Names

B-cell lymphoma 3 protein homolog, BCL-3

3 Images
Flow Cytometry - Anti-Bcl3 antibody [Ham150-3.5] (AB15046)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bcl3 antibody [Ham150-3.5] (AB15046)

Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos cells staining BCL-3 with ab15046 at 1μg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).

Flow Cytometry - Anti-Bcl3 antibody [Ham150-3.5] (AB15046)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bcl3 antibody [Ham150-3.5] (AB15046)

Flow cytometric analysis of 2% paraformaldehyde-fixed BAF3cells staining BCL-3 with ab15046 at 1μg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).

Western blot - Anti-Bcl3 antibody [Ham150-3.5] (AB15046)
  • WB

Unknown

Western blot - Anti-Bcl3 antibody [Ham150-3.5] (AB15046)

Western blot of Bcl3 in transgenic mouse T cells using ab15046.

All lanes:

Western blot - Anti-Bcl3 antibody [Ham150-3.5] (ab15046) at 2 µg/mL

Predicted band size: 48 kDa

false

Key facts

Host species

Hamster

Clonality

Monoclonal

Clone number

Ham150-3.5

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Mouse Bcl3. The exact immunogen used to generate this antibody is proprietary information.

Q9Z2F6

Specificity

ab15046 recognises Bcl3 from transfected cells and shows no cross reactivity to other Bcl isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>1 μg/test (100μl)</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>1 μg/test (100μl)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl3 protein also known as Bcl-3 is a member of the IκB family of proteins. It serves a mechanical role as a transcription coactivator that interacts with NF-κB p50 and p52 homodimers. The Bcl3 protein has a molecular weight of approximately 45 kDa. This protein expresses primarily in lymphoid tissues including spleen and lymph nodes usually found within the nucleus where it performs its function in regulating gene expression.
Biological function summary

Bcl3 interacts with specific components of the NF-κB complex and modulates signaling pathways involved in immune responses inflammation and cell proliferation. Bcl3 helps in the stabilization and activation of p50/p52 dimers which facilitate the transcription of target genes. Its role necessitates its function within larger complexes that are essential for transcriptional regulation. Bcl3's interaction with other transcription factors allows it to fine-tune the expression of a range of genes impacting several cellular processes.

Pathways

Bcl3 plays a significant role in the regulation of the NF-κB signaling pathway. It acts by modulating the activity of NF-κB dimers important for immune response and inflammation. Furthermore it affects the PI3K/Akt pathway by influencing the expression of genes that are relevant to cell survival and apoptosis. Proteins such as p50 and p52 in the NF-κB pathway closely relate to Bcl3 function highlighting its role in cellular signaling networks.

Bcl3 is linked to certain types of cancers and autoimmune diseases. Its abnormal expression associates with disorders like chronic lymphocytic leukemia where it may contribute to disease progression by affecting cell proliferation and survival. Bcl3's interaction with NF-κB components can also influence rheumatoid arthritis where it might play a part in exacerbating inflammation. Proteins such as p50 and other NF-κB pathway components connect Bcl3 to these diseases reflecting its importance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit (By similarity). In the nucleus, acts as a transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
See full target information Bcl3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 82:10042-51 PubMed18684816

2008

Bap31 is a novel target of the human papillomavirus E5 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Regan,Laimonis A Laimins
View all publications
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