AB314099

Anti-Bcl6 antibody [BLR143H] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal BCL6 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BCL6 aa 350-450.

View Alternative Names

BCL5, LAZ3, ZBTB27, ZNF51, BCL6, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL-5

5 Images

Supplier Data

Immunocytochemistry - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Detection of human Bcl6 in FFPE Raji cells by ICC. Antibody : Rabbit anti-Bcl6 recombinant monoclonal antibody [BLR143H] (A700-143). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Detection of human Bcl6 in FFPE tonsil by IHC. Antibody : Rabbit anti-Bcl6 recombinant monoclonal antibody [BLR143H] (A700-143). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Flow Cytometry - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Detection of human Bcl6 (shaded) in Ramos cells by flow cytometry. Antibody : Rabbit anti-Bcl6 recombinant monoclonal [BLR143H] (A700-143) or isotype control (unshaded). Secondary : 488-conjugated goat anti-rabbit IgG.

Supplier Data

Immunoprecipitation - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Samples : Whole cell lysate (1 mg for IP; 5% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-Bcl6 recombinant monoclonal antibody [BLR143H] (A700-143) used for IP at 6 µl per reaction. Bcl6 was also immunoprecipitated by rabbit anti-Bcl6 antibody BL-1481A-1F2. For blotting immunoprecipitated Bcl6, A700-143 was used at 1 : 1000. Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Bcl6 antibody [BLR143H] - BSA free (ab314099) at 1/1000 dilution

All lanes:

Ramos cells at 1 mg

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Supplier Data

Western blot - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Samples : Whole cell lysate (5 µg) from Ramos, Daudi, Raji, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Bcl6 recombinant monoclonal antibody [BLR143H] (A700-143) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-Bcl6 antibody [BLR143H] - BSA free (ab314099) at 1/1000 dilution

Lane 1:

Ramos Whole cell lysate at 5 µg

Lane 2:

Daudi Whole cell lysate at 5 µg

Lane 3:

Raji Whole cell lysate at 5 µg

Lane 4:

HEK293T Whole cell lysate at 5 µg

Lane 5:

HeLa Whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR143H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, WB, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human BCL6 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P41182

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl6 protein also known as B-cell lymphoma 6 is a transcriptional repressor important for the germinal center formation in B-cells. It has a molecular weight of approximately 95 kDa. Bcl6 is expressed mainly in B-cells T-cells and certain subsets of dendritic cells. It functions by binding to target DNA sequences where it represses the expression of specific genes leading to controlled cell proliferation differentiation and apoptosis. Researchers often study Bcl6 through the use of monoclonal antibodies particularly in immunohistochemistry (IHC).

Biological function summary

Bcl6 plays essential roles in immune response regulation and lymphocyte development. In germinal centers Bcl6 influences the process of somatic hypermutation and class switch recombination in B-cells which is a part of generating antibody diversity. It operates as a central player in a multiprotein complex that includes corepressors such as SMRT and NCoR which facilitate the repression of its target genes. This regulatory action helps maintain the precise balance of immune cell functions.

Pathways

Bcl6 integrates into the B-cell receptor (BCR) signaling and the JAK-STAT pathway. It interacts with molecules like STAT1 and STAT3 positively influencing the survival and proliferation of B-cells. In these pathways Bcl6's activity modulates the transcriptional landscape that determines cell fate decisions affecting cellular processes such as proliferation and survival in response to external cues.

Bcl6 is relevant to B cell lymphomas specifically diffuse large B-cell lymphoma (DLBCL) where it is often overexpressed contributing to malignancy by preventing cell death. This pathology frequently involves chromosomal translocations that juxtapose the Bcl6 gene with immunoglobulin genes. The relationship between Bcl6 and other proteins like Bcl2 is noteworthy in this context as Bcl2 inhibits apoptosis further contributing to cancer progression. Mutations and dysregulated expression of Bcl6 have also shown links to autoimmune diseases where its role in cell differentiation and survival may lead to aberrant immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

See full target information BCL6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl6 antibody [BLR143H] - BSA free

Immunocytochemistry - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Flow Cytometry - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Immunoprecipitation - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Western blot - Anti-Bcl6 antibody [BLR143H] - BSA free (AB314099)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise