AB172610

Anti-Bcl6 antibody [EPR11410-43]

Name: Anti-Bcl6 antibody [EPR11410-43] Rabbit monoclonal (ab172610) | Abcam
Brand: Abcam Limited
SKU: AB172610
Price: 405 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(3 Reviews)

(14 Publications)

Rabbit Recombinant Monoclonal BCL6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 14 publications.

View Alternative Names

BCL5, LAZ3, ZBTB27, ZNF51, BCL6, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL-5

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node in stomach tissue sections labeling bcl-6 with purified ab172610 at 1 : 1500 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

IHC-P

AbReview63529****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

ab172610 staining bcl-6 in Human tonsil tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with 1% PB ab64226 for 10 minutes at room tempreture and antigen retrieval was by heat mediation in EDTA pH 9. The sample was incubated with primary antibody (1/100 in Leica Bond Diluent) at for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (undiluted) was used as the secondary antibody. This image was produced using unpurified antibody.

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

All lanes:

Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/2000 dilution

All lanes:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 20000 µg

Predicted band size: 79 kDa

Observed band size: 78 kDa

false

Supplier Data

Western blot - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Predicted band size: 79 kDa

false

Carrier free

Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free
Biotin

Biotin Anti-Bcl6 antibody [EPR11410-43]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11410-43

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1500", "IHCP-species-notes": "For unpurified use at 1/200 - 1/500. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl6 protein also known as B-cell lymphoma 6 is a transcriptional repressor important for the germinal center formation in B-cells. It has a molecular weight of approximately 95 kDa. Bcl6 is expressed mainly in B-cells T-cells and certain subsets of dendritic cells. It functions by binding to target DNA sequences where it represses the expression of specific genes leading to controlled cell proliferation differentiation and apoptosis. Researchers often study Bcl6 through the use of monoclonal antibodies particularly in immunohistochemistry (IHC).

Biological function summary

Bcl6 plays essential roles in immune response regulation and lymphocyte development. In germinal centers Bcl6 influences the process of somatic hypermutation and class switch recombination in B-cells which is a part of generating antibody diversity. It operates as a central player in a multiprotein complex that includes corepressors such as SMRT and NCoR which facilitate the repression of its target genes. This regulatory action helps maintain the precise balance of immune cell functions.

Pathways

Bcl6 integrates into the B-cell receptor (BCR) signaling and the JAK-STAT pathway. It interacts with molecules like STAT1 and STAT3 positively influencing the survival and proliferation of B-cells. In these pathways Bcl6's activity modulates the transcriptional landscape that determines cell fate decisions affecting cellular processes such as proliferation and survival in response to external cues.

Bcl6 is relevant to B cell lymphomas specifically diffuse large B-cell lymphoma (DLBCL) where it is often overexpressed contributing to malignancy by preventing cell death. This pathology frequently involves chromosomal translocations that juxtapose the Bcl6 gene with immunoglobulin genes. The relationship between Bcl6 and other proteins like Bcl2 is noteworthy in this context as Bcl2 inhibits apoptosis further contributing to cancer progression. Mutations and dysregulated expression of Bcl6 have also shown links to autoimmune diseases where its role in cell differentiation and survival may lead to aberrant immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunoglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

See full target information BCL6

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013402 PubMed40794649

2025

Age-associated immune dysregulation and B cell dysfunction drive severe outcomes in SFTSV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ha Kim,Ho Bin Jang,Se-Mi Kim,Dongbin Park,Young-Il Kim,Seung-Gyu Jang,Suhee Hwang,Woo-Hyun Kwon,Isaac Choi,Jaemoo Kim,Hee-Dong Yang,Hyunjoon Kim,Mark Anthony B Casel,Min-Suk Song,Jae U Jung,Young Ki Choi

Frontiers in pharmacology 16:1634995 PubMed40777995

2025

BCL6 promotes the progression of high-grade serous ovarian cancer cells by inhibiting PLAAT4.

Applications

Unspecified application

Species

Unspecified reactive species

An Wan,Wei-Dong Zhao,Gang Chen,Cheng Peng,Jin-Hui Tao

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

Transplantation 106:1974-1989 PubMed35442232

2022

Ferret Lung Transplantation Models Differential Lymphoid Aggregate Morphology Between Restrictive and Obstructive Forms of Chronic Lung Allograft Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Lynch,Bethany A Ahlers,Anthony M Swatek,Vitaly Ievlev,Albert C Pai,Leonard Brooks,Yinghua Tang,Idil A Evans,David K Meyerholz,John F Engelhardt,Kalpaj R Parekh

BioFactors (Oxford, England) 47:386-402 PubMed33502806

2021

BCL-6 promotes the methylation of miR-34a by recruiting EZH2 and upregulating CTRP9 to protect ischemic myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiunn-Miin Lin,Chih-Hsiang Hsu,Jeen-Chen Chen,Shao-Hsuan Kao,You-Cian Lin

BioMed research international 2020:7467104 PubMed33376737

2020

Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor Alpha/B-Cell Lymphoma 6 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Li,Dandan Meng,Yue Xu,Ling Gao,Fengqian Shen,Xiaojing Tie,Yan Zhang,Zhenying Yi,Wenjie Shen,Zonglan Liu,Zhiqiao Xu

Oncology letters 20:285 PubMed33014163

2020

Isolated oculomotor nerve palsy as a manifestation of diffuse large B cell lymphoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Muntadher M Khaleefah,Siddharth Narayanan,Hiba A Al Dallal,Christopher M Jones,Robert P Friedland,Adriana E Palade,Kerri S Remmel,Jignesh J Shah

Cancer cell 37:674-689.e12 PubMed32330455

2020

Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Elie Dheilly,Elena Battistello,Natalya Katanayeva,Stephanie Sungalee,Justine Michaux,Gerben Duns,Sarah Wehrle,Jessica Sordet-Dessimoz,Marco Mina,Julien Racle,Pedro Farinha,George Coukos,David Gfeller,Anja Mottok,Robert Kridel,Bruno E Correia,Christian Steidl,Michal Bassani-Sternberg,Giovanni Ciriello,Vincent Zoete,Elisa Oricchio

Oncology letters 19:2097-2106 PubMed32194707

2020

Curative effect of methotrexate combined with teniposide in the treatment of primary central nervous system lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Xia Wang,Yan Huang,Xiao-Ping Xu,Bo-Bin Chen,Zhi-Guang Lin,Yan Ma,Tian-Ling Ding,Qian Wang

Proteomics. Clinical applications 14:e1900091 PubMed31721454

2019

Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Suzuki,Atsushi Muroi,Masanori Nojima,Ayumi Numata,Hirotaka Takasaki,Rika Sakai,Tomoyuki Yokose,Yohei Miyagi,Naohiko Koshikawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcl6 antibody [EPR11410-43]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Western blot - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

Western blot - Anti-Bcl6 antibody [EPR11410-43] (AB172610)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise