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Mouse Recombinant Monoclonal BCL6 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (AB252801), expandable thumbnail
  • Immunoprecipitation - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (AB252801), expandable thumbnail
  • Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (AB252801), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (AB252801), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (AB252801), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Not recommended
Tested
Rat
Expected
Not recommended
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
13.433 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.403 µg/mL
Notes

-

Species
Human
Dilution info
0.403 µg/mL
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Alternative names

BCL5, LAZ3, ZBTB27, ZNF51, BCL6, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL-5

Recommended products

Mouse Recombinant Monoclonal BCL6 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
GI191E
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab252801 is the carrier-free version of Anti-Bcl6 antibody [GI191E] ab241549.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Bcl6 protein also known as B-cell lymphoma 6 is a transcriptional repressor important for the germinal center formation in B-cells. It has a molecular weight of approximately 95 kDa. Bcl6 is expressed mainly in B-cells T-cells and certain subsets of dendritic cells. It functions by binding to target DNA sequences where it represses the expression of specific genes leading to controlled cell proliferation differentiation and apoptosis. Researchers often study Bcl6 through the use of monoclonal antibodies particularly in immunohistochemistry (IHC).

Biological function summary

Bcl6 plays essential roles in immune response regulation and lymphocyte development. In germinal centers Bcl6 influences the process of somatic hypermutation and class switch recombination in B-cells which is a part of generating antibody diversity. It operates as a central player in a multiprotein complex that includes corepressors such as SMRT and NCoR which facilitate the repression of its target genes. This regulatory action helps maintain the precise balance of immune cell functions.

Pathways

Bcl6 integrates into the B-cell receptor (BCR) signaling and the JAK-STAT pathway. It interacts with molecules like STAT1 and STAT3 positively influencing the survival and proliferation of B-cells. In these pathways Bcl6's activity modulates the transcriptional landscape that determines cell fate decisions affecting cellular processes such as proliferation and survival in response to external cues.

Associated diseases and disorders

Bcl6 is relevant to B cell lymphomas specifically diffuse large B-cell lymphoma (DLBCL) where it is often overexpressed contributing to malignancy by preventing cell death. This pathology frequently involves chromosomal translocations that juxtapose the Bcl6 gene with immunoglobulin genes. The relationship between Bcl6 and other proteins like Bcl2 is noteworthy in this context as Bcl2 inhibits apoptosis further contributing to cancer progression. Mutations and dysregulated expression of Bcl6 have also shown links to autoimmune diseases where its role in cell differentiation and survival may lead to aberrant immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Bcl6 antibody [GI191E] ab241549).

    All lanes: Western blot - Anti-Bcl6 antibody [GI191E] (Anti-Bcl6 antibody [GI191E] ab241549) at 1/1000 dilution

    Lane 1: Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 79 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Immunoprecipitation - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation Anti-Bcl6 antibody [GI191E] ab241549, PBS, sodium azide, glycerol and BSA.

    Bcl6 was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with Anti-Bcl6 antibody [GI191E] ab241549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Bcl6 antibody [GI191E] ab241549 at 1/500 dilution. Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/1000 dilution.

    Lane 1: Ramos whole cell lysate 10μg (Input).
    Lane 2: Anti-Bcl6 antibody [GI191E] ab241549 IP in Ramos whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of Anti-Bcl6 antibody [GI191E] ab241549 in Ramos whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-Bcl6 antibody [GI191E] (Anti-Bcl6 antibody [GI191E] ab241549)

    Predicted band size: 79 kDa

  • Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Bcl6 antibody [GI191E] ab241549).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1,3: 81 secs; Lane 2: 5 secs.

    All lanes: Western blot - Anti-Bcl6 antibody [GI191E] (Anti-Bcl6 antibody [GI191E] ab241549) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 79 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Bcl6 antibody [GI191E] ab241549).

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Bcl6 with Anti-Bcl6 antibody [GI191E] ab241549 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nuclear staining on the germinal center in the human tonsil. The section was incubated with Anti-Bcl6 antibody [GI191E] ab241549 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

    The section was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Bcl6 antibody [GI191E] ab241549).

    Immunohistochemical analysis of paraffin-embedded Rat mesenteric lymph nodes tissue labelling Bcl6 with Anti-Bcl6 antibody [GI191E] ab241549 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nuclear staining on the germinal center in the rat mesenteric lymph nodes. The section was incubated with Anti-Bcl6 antibody [GI191E] ab241549 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

    The section was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Bcl6 antibody [GI191E] ab241549).

    Immunohistochemical analysis of paraffin-embedded Mouse mesenteric lymph nodes tissue labelling Bcl6 with Anti-Bcl6 antibody [GI191E] ab241549 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nuclear staining on the germinal center in the mouse mesenteric lymph nodes. The section was incubated with Anti-Bcl6 antibody [GI191E] ab241549 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

    The section was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Bcl6 antibody [GI191E] ab241549).

    Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labelling Bcl6 with Anti-Bcl6 antibody [GI191E] ab241549 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Nuclear staining on the human B cell lymphoma. The section was incubated with Anti-Bcl6 antibody [GI191E] ab241549 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

    The section was counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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