Anti-Bcl6 antibody [K112-91] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [K112-91] - BSA and Azide free (AB307682)

This data was produced using ab307681, the same antibody clone but in a different buffer. Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl6 with ab307681 at 1/1000 (0.992 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human tonsil. The section was incubated with ab307681 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [K112-91] - BSA and Azide free (AB307682)

This data was produced using ab307681, the same antibody clone but in a different buffer. Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's tissue labeling Bcl6 with ab307681 at 1/1000 (0.992 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human non-Hodgkin's B-cell lymphoma. The section was incubated with ab307681 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Bcl6 antibody [K112-91] - BSA and Azide free (AB307682)

This data was produced using ab307681, the same antibody clone but in a different buffer. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling Bcl6 with ab307681 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Negative control : Jurkat (PMID : 8652841, 10757802).

• WB

Supplier Data

Western blot - Anti-Bcl6 antibody [K112-91] - BSA and Azide free (AB307682)

This data was produced using ab307681, the same antibody clone but in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Jurkat (PMID : 8652841, 10757802). The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 59 seconds.

All lanes:

Western blot - Anti-Bcl6 antibody [K112-91] (<a href='/en-us/products/primary-antibodies/bcl6-antibody-k112-91-ab307681'>ab307681</a>) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 78 kDa

false

Exposure time: 59s