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Rabbit Recombinant Multiclonal BCL6 antibody. Suitable for WB, IP, IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Bcl6 antibody [RM1183] (AB317337), expandable thumbnail
  • Western blot - Anti-Bcl6 antibody [RM1183] (AB317337), expandable thumbnail
  • Western blot - Anti-Bcl6 antibody [RM1183] (AB317337), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (AB317337), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (AB317337), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Alternative names

BCL5, LAZ3, ZBTB27, ZNF51, BCL6, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL-5

Recommended products

Rabbit Recombinant Multiclonal BCL6 antibody. Suitable for WB, IP, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM1183
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Bcl6 protein also known as B-cell lymphoma 6 is a transcriptional repressor important for the germinal center formation in B-cells. It has a molecular weight of approximately 95 kDa. Bcl6 is expressed mainly in B-cells T-cells and certain subsets of dendritic cells. It functions by binding to target DNA sequences where it represses the expression of specific genes leading to controlled cell proliferation differentiation and apoptosis. Researchers often study Bcl6 through the use of monoclonal antibodies particularly in immunohistochemistry (IHC).

Biological function summary

Bcl6 plays essential roles in immune response regulation and lymphocyte development. In germinal centers Bcl6 influences the process of somatic hypermutation and class switch recombination in B-cells which is a part of generating antibody diversity. It operates as a central player in a multiprotein complex that includes corepressors such as SMRT and NCoR which facilitate the repression of its target genes. This regulatory action helps maintain the precise balance of immune cell functions.

Pathways

Bcl6 integrates into the B-cell receptor (BCR) signaling and the JAK-STAT pathway. It interacts with molecules like STAT1 and STAT3 positively influencing the survival and proliferation of B-cells. In these pathways Bcl6's activity modulates the transcriptional landscape that determines cell fate decisions affecting cellular processes such as proliferation and survival in response to external cues.

Associated diseases and disorders

Bcl6 is relevant to B cell lymphomas specifically diffuse large B-cell lymphoma (DLBCL) where it is often overexpressed contributing to malignancy by preventing cell death. This pathology frequently involves chromosomal translocations that juxtapose the Bcl6 gene with immunoglobulin genes. The relationship between Bcl6 and other proteins like Bcl2 is noteworthy in this context as Bcl2 inhibits apoptosis further contributing to cancer progression. Mutations and dysregulated expression of Bcl6 have also shown links to autoimmune diseases where its role in cell differentiation and survival may lead to aberrant immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Immunoprecipitation - Anti-Bcl6 antibody [RM1183] (ab317337)

    Bcl6 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab317337 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317337 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lane 2: ab317337 IP in Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317337 in Ramos whole cell lysate

    All lanes: Immunoprecipitation - Anti-Bcl6 antibody [RM1183] (ab317337) at 1/30 dilution

    All lanes: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Western blot - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)

    Low expression: 293T

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Bcl6 antibody [RM1183] (ab317337) at 1/1000 dilution

    Lane 1: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 78 kDa, 36 kDa

    Exposure time: 10s

  • Western blot - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)

    Low expression: Jurkat (PMID: 8652841)

    This antibody was tested on fresh and frozen Romas and Jurkat Whole Cell Lysate.

    The Lysates in lanes 3-4 were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Bcl6 antibody [RM1183] (ab317337) at 1/1000 dilution

    Lane 1: Ramos (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: Ramos whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: Jurkat whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 78 kDa, 36 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human liver. The section was incubated with ab317337 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human cerebrum. The section was incubated with ab317337 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)

    Immunohistochemical analysis of paraffin-embedded Human non-hodgkin's lymphoma tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human non-hodgkin's lymphoma. The section was incubated with ab317337 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)

    Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human diffuse large B-cell lymphoma. The section was incubated with ab317337 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human tonsil. The section was incubated with ab317337 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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